@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001417, author = "Yao, Su and Zhai, Lei and Xin, Chunhui and Liu, Yang and Xu, Ling and Zhang, Xin and Zhao, Ting and Zhang, Lu and Cheng, Chi", title = "Scopulibacillus daqui sp. nov., a thermophilic bacterium isolated from high temperature daqu", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "11", pages = "4723-4728", doi = "https://doi.org/10.1099/ijsem.0.001417", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001417", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "high temperature daqu", keywords = "Scopulibacillus daqui", abstract = "A Gram-stain-positive, facultatively anaerobic, thermophilic bacterium, designated ZQ18-1T, was isolated from a high temperature daqu sample collected from the sesame-flavour liquor-making process. Oval endospores were formed at the centre of cells with swollen sporangia. The isolate was able to grow at temperatures of 20–60 °C (optimum growth at 50 °C), at pH 4–9 (optimum growth at pH 8) and in the presence of 0–10 % (w/v) NaCl (optimum growth with 2 % NaCl). Glucose and galactose were major cell-wall sugars, and meso-diaminopimelic acid was the diagnostic amino acid. The major polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and three glycolipids. The major cellular fatty acids were anteiso-C17 : 0 and iso-C16 : 0, and the predominant menaquinone was MK-7. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain ZQ18-1T was most closely related to Scopulibacillus darangshiensis DLS-06T, Pullulanibacillus pueri YN3T, Tuberibacillus calidus 607T, Pullulanibacillus naganoensis ATCC 53909T, Pullulanibacillus uraniitolerans UG-2T, Sporolactobacillus terrae DSM 11697T and Sporolactobacillus inulinus NRIC 1133T. Strain ZQ18-1T showed low DNA–DNA relatedness (40.7, 23.1, 46.5, 27.2, 45.6, 33.7 and 55.1 %) with the strains mentioned above. Based on morphological characteristics, chemotaxonomic characteristics, DNA–DNA hybridization data and physiological properties, strain ZQ18-1T represents a novel species of the genus Scopulibacillus , for which the name Scopulibacillus daqui sp. nov. is proposed. The type strain is Scopulibacillus daqui ZQ18-1T (=DSM 28236T=CICC 10824T). An emended description of the genus Scopulibacillus is provided.", }