RT Journal Article SR Electronic(1) A1 Korshunova, Tatiana Y. A1 Ramírez-Bahena, Martha-Helena A1 Chetverikov, Sergey P. A1 Igual, Jose M. A1 Peix, Álvaro A1 Loginov, OlegYR 2016 T1 Pseudomonas turukhanskensis sp. nov., isolated from oil-contaminated soils JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 11 SP 4657 OP 4664 DO https://doi.org/10.1099/ijsem.0.001406 PB Microbiology Society, SN 1466-5034, AB A bacterial strain named IB1.1T was isolated in a screening of hydrocarbon-degrading bacteria from oil-contaminated soils on the territory of the Turukhansk District of Krasnoyarsk Krai, East Siberia, Russia. The 16S rRNA gene sequence had 98.7 % identity with respect to the closest phylogenetic relative, Pseudomonas granadensis F-278,770T, and the next most closely related species with 98.6 % similarity was Pseudomonas punonensis , suggesting that IB1.1T should be classified within the genus Pseudomonas . The analysis of housekeeping genes rpoB, rpoD and gyrB showed similarities lower than 90 % in all cases with respect to the closest relatives, confirming its phylogenetic affiliation. The strain showed a polar flagellum. The respiratory quinone was Q9. The major fatty acids were 16 : 1ω7c/16 : 1ω6c (summed feature 3), 18 : 1ω7c and 16 : 0. The strain was oxidase- and catalase-positive, but the arginine dihydrolase system was not present. Nitrate reduction, urease and β–galactosidase production, and aesculin hydrolysis were negative. The temperature range for growth was 4–34 °C, and the strain could grow at pH 11. The DNA G+C content was 58.5 mol%. DNA–DNA hybridization results showed values of less than 30 % relatedness with respect to the type strains of the eight most closely related species. Therefore, the dataset of genotypic, phenotypic and chemotaxonomic data support the classification of strain IB1.1T into a novel species of the genus Pseudomonas , for which the name Pseudomonas turukhanskensis sp. nov. is proposed. The type strain is IB1.1T (=VKM B-2935T=CECT 9091T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001406