1887

Abstract

A novel actinomycete strain, designated strain KLBMP S0027, was isolated from a coastal soil collected from the coastal region of Lianyungang, Jiangsu Province, in east China, and was studied in detail for its taxonomic position. Comparative 16S rRNA gene sequence analysis showed that this strain belonged to the genus and was most closely related to WS-26 (98.5 %), NBRC 100129 (98.5 %), NBRC 100374 (98.2 %), NBRC 14402 (98.2 %) and NBRC 100375 (98 %), respectively. The strain showed a combination of chemotaxonomic and morphological properties typical of the genus . The cell wall contained -diaminopimelic acid (type IV), and whole-cell sugars were arabinose, galactose, glucose and ribose. Strain KLBMP S0027 contained MK-8(Hω-cycl) as the predominant menaquinone; C, Cω9, C 10-methyl (TBSA) and summed feature 3 (Cω7/Cω6) were the major cellular fatty acids. The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unknown glycolipid and two unknown lipids. Mycolic acids were detected. The G+C content of the DNA was 70.5 %. However, a combination of DNA–DNA hybridization and phenotypic data demonstrated that strain KLBMP S0027 could be clearly distinguished from the type strain of the most closely related species, WS-26. On the basis of the data presented from a polyphasic study, it was evident that this strain should be assigned to a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KLBMP S0027 (=CGMCC 4.7330=KCTC 39691).

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2016-11-01
2020-01-27
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