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Abstract

A novel Gram-stain-negative, rod-shaped, non-spore-forming, non-flagellated, strictly aerobic strain, designated RZW3-2, was isolated from seawater from near the Yellow Sea coast of PR China (35.475° N 119.613° E). The organism grew optimally between 24 and 28 °C, at pH 6.0–7.0 and in the presence of 2–3 % (w/v) NaCl. The strain requires seawater or artificial seawater for growth and NaCl alone does not support growth. RZW3-2 contained iso-C, Cω7 and/or Cω6 and anteiso-C as the dominant fatty acids. The respiratory quinone detected in RZW3-2 was menaquinone 6 (MK6). The polar lipids of RZW3-2 comprised phosphatidylethanolamine (PE), four unidentified phospholipids (PL), two unidentified aminolipids (AL) and one unknown lipid (L). The DNA G+C content of RZW3-2 was 30.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to KCTC 23969, KCTC 12392 and CIP 106478 with 98.0, 97.8 and 97.0 % sequence similarities, respectively. The DNA–DNA hybridization values between RZW3-2 and its closest phylogenetic relatives, KCTC 23969 and KCTC 12392, were 52.0±0.6 % and 49.8±1.21 % respectively. On the basis of polyphasic analyses, RZW3-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RZW3-2 (=JCM 30825=KCTC 42664=MCCC 1K00696).

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2016-11-01
2022-01-21
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