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Abstract

A bacterial strain, designated TTM-2, was isolated from a water sample taken from the Caohu River in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain TTM-2 were Gram-stain-negative, aerobic, poly-β-hydroxybutyrate-accumulating, non-motile, rod-shaped and covered by large capsules; they formed light pink colonies. Growth occurred at 15–37 °C (optimum, 30 °C), at pH 4–8 (optimum, pH 6) and with 0–1 % (w/v) NaCl (optimum, 0.5 %, w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain TTM-2 belonged to the genus . The sequence similarities between the 16S rRNA genes of strain TTM-2 and the type strains of other species of the genus ranged from 93.6 to 97.4 %. The closest relatives of strain TTM-2 were R9-65 (97.4 %) and A5 (97.1 %). The predominant fatty acids were iso-C (36.9 %) and summed feature 3 (Cω7 and/or Cω6; 33.7 %). The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was MK-7. The DNA G+C content of the genomic DNA was 46.6 mol%. The DNA-DNA relatedness of strain TTM-2 with respect to species of the genus with validly published names was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain TTM-2 should be classified as a novel species, for which the name sp. nov. is proposed. The type strain is TTM-2 (=BCRC 80785=LMG 28455=KCTC 42274).

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2016-11-01
2019-12-12
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