1887

Abstract

A Gram-stain-negative, non-motile, light-pink-pigmented, aerobic, rod-shaped bacterium, designated V5RD, was isolated from soil of Damxung county in the Qinghai-Tibetan Plateau. Strain V5RD grew luxuriously at 10 °C, at pH 9.0 and in the presence of 1 % NaCl (w/v). Phylogenetic analysis of 16S rRNA gene sequences placed strain V5RD in the genus Pedobacter and found that it was most closely related to Pedobacter alluvionis DSM 19624 (97.3 %), Pedobacter ginsenosidimutans JCM 16721 (96.84 %), Pedobacter agri DSM 19486 (96.28 %), Pedobacter roseus JCM 13399 (96.22 %), Pedobacter sandarakinus KCTC 12559 (95.92 %) and Pedobacter borealis DSM 19626 (95.85 %). The G+C content of the genomic DNA of the type strain V5RD was 37.40 mol%. DNA–DNA relatedness for the type strain V5RD with respect to its closest phylogenetic relative, P . alluvionis DSM 19624, was 62.5±1.7 %. The polar lipid profile of the strain consisted of phosphatidylethanolamine, one unidentified aminolipid, one unidentified glycolipid and two unidentified polar lipids. Menaquinone MK-7 was the predominant respiratory quinone, and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH were the major fatty acids. With respect to phenotypic characteristics, biochemical properties and phylogenetic inference, strain V5RD represents a novel species of the genus Pedobacter , for which the name Pedobacter psychrotolerans sp. nov is proposed. The type strain is V5RD (=CGMCC 1.15644=DSM 103236).

Keyword(s): Nobel species , Soil and Tibetan Plateau
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2016-11-01
2019-10-14
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