Chelatococcus reniformis sp. nov., isolated from a glacier

Zhengquan Gu; Yongqin Liu; Ninglian Wang; Nianzhi Jiao; Liang Shen; Hongcan Liu; Yuguang Zhou; Xiaobo Liu; Jiule Li; Jingnan Liang; Hans-Jürgen Busse

doi:10.1099/ijsem.0.001384

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Chelatococcus reniformis sp. nov., isolated from a glacier

Zhengquan Gu¹, Yongqin Liu^1,2, Ninglian Wang^2,3, Nianzhi Jiao⁴, Liang Shen¹, Hongcan Liu⁵, Yuguang Zhou⁵, Xiaobo Liu¹, Jiule Li¹, Jingnan Liang⁶, Hans-Jürgen Busse⁷
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Affiliations: ¹ Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, PR China ² CAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing 100085, PR China ³ Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, PR China ⁴ State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, PR China ⁵ Institute of Microbiology, China General Microbiological Culture Collection Center, Chinese Academy of Sciences, Beijing 100101, PR China ⁶ Institute of Microbiology, Instrument Center, Chinese Academy of Sciences, Beijing 100101, PR China ⁷ Institut für Mikrobiologie Veterinärmedizinische, Universität Wien Veterinärplatz, Wien 1A-1210, Austria
Correspondence Yongqin Liu [email protected]
Published: 01 November 2016 https://doi.org/10.1099/ijsem.0.001384

Abstract

A Gram-stain-negative, non-motile, reniform bacterial strain, B2974T, was isolated from an ice core of the Muztagh Glacier, on the Tibetan Plateau, China. Strain B2974T grew optimally at pH 7.0–7.5 and 25–30 °C in the presence of 0–2.0 % (w/v) NaCl. 16S rRNA gene sequence similarity analysis indicated that strain B2974T was closely related to Chelatococcus asaccharovorans LMG 25503T at a level of 97.1 %. The major quinone of strain B2974T was ubiquinone Q10. The predominant fatty acids were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C19 : 0 cyclo ω8c. sym-Homospermidine was the major polyamine. The genomic DNA G+C content of the strain was 64 mol%. In DNA–DNA hybridization tests, strain B2974T shared 49.32 % DNA–DNA relatedness with the type strain of Chelatococcus asaccharovorans LMG 25503T. Based on the results of phenotypic and chemotaxonomic characteristics, strain B2974T was considered as a novel species of the genus Chelatococcus , for which the name Chelatococcus reniformis sp. nov. is proposed. The type strain is B2974T (=JCM 30308T=CGMCC 1.12919T).

Received: 23/05/2016
Accepted: 03/08/2016
Published Online: 01/11/2016

Keyword(s): Chelatococcus sp. nov. and from glacier

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Chelatococcus reniformis sp. nov., isolated from a glacier

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Chelatococcus reniformis sp. nov., isolated from a glacier

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