1887

Abstract

A Gram-stain-negative, non-motile, reniform bacterial strain, B2974, was isolated from an ice core of the Muztagh Glacier, on the Tibetan Plateau, China. Strain B2974 grew optimally at pH 7.0–7.5 and 25–30 °C in the presence of 0–2.0 % (w/v) NaCl. 16S rRNA gene sequence similarity analysis indicated that strain B2974 was closely related to LMG 25503 at a level of 97.1 %. The major quinone of strain B2974 was ubiquinone Q10. The predominant fatty acids were summed feature 8 (C 7 and/or C ) and C cyclo 8. -Homospermidine was the major polyamine. The genomic DNA G+C content of the strain was 64 mol%. In DNA–DNA hybridization tests, strain B2974 shared 49.32 % DNA–DNA relatedness with the type strain of LMG 25503. Based on the results of phenotypic and chemotaxonomic characteristics, strain B2974 was considered as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B2974 (=JCM 30308=CGMCC 1.12919).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001384
2016-11-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4525.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001384&mimeType=html&fmt=ahah

References

  1. Auling G., Busse H.-J., Egli T., El-Banna T., Stackebrandt E. 1993; Description of the Gram-negative, obligately aerobic, nitrilotriacetate (NTA)-utilizing bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov. Syst Appl Microbiol 16:104–112 [View Article]
    [Google Scholar]
  2. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology , pp. 137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Busse J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the proteobacteria. Syst Appl Microbiol 11:1–8 [View Article]
    [Google Scholar]
  4. Busse H.-J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [View Article]
    [Google Scholar]
  5. De Ley J. D., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  6. Egli T. W., Auling G. 2005; Genus Chelatococcus. In Bergey’s Manual of Systematic Bacteriology, Part C, 2nd edn. vol. 2 , pp. 433–437 Edited by Brenner D. J., Krieg N. R., Stanley J. T. New York: Springer; [CrossRef]
    [Google Scholar]
  7. Embley T. M. 1991; The linear PCR reaction: a simple and robust method for sequencing amplified rRNA genes. Lett Appl Microbiol 13:171–174 [View Article][PubMed]
    [Google Scholar]
  8. Hiraishi A., Ueda Y., Ishihara J. 1998; Quinone profiling of bacterial communities in natural and synthetic sewage activated sludge for enhanced phosphate removal. Appl Environ Microbiol 64:992–998[PubMed]
    [Google Scholar]
  9. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  10. Jin L., Ko S.-R., Lee H.-G., Kim B.-H., Kim H.-S., Ahn C.-Y., Oh H.-M. 2015; Chelatococcus caeni sp. nov., isolated from sludge sample of a biofilm reactor. Int J Syst Evol Microbiol. 65:885–889 [CrossRef]
    [Google Scholar]
  11. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  12. Kämpfer P., Scholz H. C., Langer S., Wernery U., Wernery R., Johnson B., Joseph M., Lodders N., Busse H. J. 2010; Camelimonas lactis gen. nov., sp. nov., isolated from the milk of camels. Int J Syst Evol Microbiol 60:2382–2386 [View Article][PubMed]
    [Google Scholar]
  13. Kämpfer P., Scholz H. C., Lodders N., Loncaric I., Whatmore A. M., Busse H. J. 2012; Camelimonas abortus sp. nov., isolated from placental tissue of cattle. Int J Syst Evol Microbiol 62:1117–1120 [View Article][PubMed]
    [Google Scholar]
  14. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [View Article]
    [Google Scholar]
  15. Panday D., Das S. K. 2010; Chelatococcus sambhunathii sp. nov., a moderately thermophilic alphaproteobacterium isolated from hot spring sediment. Int J Syst Evol Microbiol 60:861–865 [View Article][PubMed]
    [Google Scholar]
  16. Reasoner D. J., Geldreich E. E. 1985; A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49:1–7[PubMed]
    [Google Scholar]
  17. Smibert R. M, Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  19. Yoon J. H., Kang S. J., Im W. T., Lee S. T., Oh T. K. 2008; Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus. Int J Syst Evol Microbiol 58:2224–2228 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001384
Loading
/content/journal/ijsem/10.1099/ijsem.0.001384
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error