@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001379, author = "Fidalgo, Cátia and Riesco, Raúl and Henriques, Isabel and Trujillo, Martha E. and Alves, Artur", title = "Microbacterium diaminobutyricum sp. nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "11", pages = "4492-4500", doi = "https://doi.org/10.1099/ijsem.0.001379", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001379", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "taxonomy", keywords = "endophytic", keywords = "halophytes", keywords = "Actinobacteria", keywords = "salt-marsh", abstract = "Three actinobacterial strains were isolated from roots of the salt-marsh plant Halimione portulacoides collected in Ria de Aveiro, Portugal. Molecular typing using enterobacterial repetitive intergenic consensus ERIC-PCR fingerprinting showed the strains to be highly similar. Phylogenetic analyses based on the 16S rRNA gene sequence and multilocus sequence analysis (MLSA) using gyrB, rpoB, recA and ppk and 16S rRNA genes sequences showed that the strains represented a member of the genus Microbacterium , with Microbacterium lacus DSM 18910T as the closest phylogenetic relative. DNA–DNA hybridization between strain RZ63T and its closest relative was below 70 %, supporting the hypothesis that it represented a distinct genomic species. Chemotaxonomic analyses of the novel strains and their DNA G+C contents confirmed their affiliation to the genus Microbacterium , however, the peptidoglycan of RZ63T contained diaminobutyric acid as the diagnostic diamino acid. In addition, physiological and fatty acid analyses revealed differences between these strains and their phylogenetic relatives, reinforcing their status as a distinct species. Based on the physiological, genetic and chemotaxonomic characterisation it is proposed that the strains studied represent a novel species of the genus Microbacterium for which the name Microbacterium diaminobutyricum sp. nov. is proposed (type strain RZ63T=DSM 27101T=CECT 8355T).", }