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Abstract

A novel Gram-stain-positive, endospore-forming bacterium, designated FJAT-22460, was isolated from a soil sample of a potato field in Xinjiang Autonomous Region, China. Cells were rods that were catalase-positive and motile by peritrichous flagella. The strain was found to grow at temperatures ranging from 10 to 40 °C (optimum 30 °C) and at pH 5.0–12.0 (optimum pH 7) with 0–5 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-22460 belonged to the genus and exhibited 16S rRNA gene sequence similarities of 97.3, 97.2, 97.2 and 97.0 % with DSM 5162, DSM 3035, MB 1871 and JCM 9905, respectively. DNA–DNA relatedness of strain FJAT-22460 with DSM 5162 and DSM 3035 was 62.6 % and 33.3 %, respectively, lower than the 70 % accepted for species delineation. The menaquinone was identified as MK-7. The major fatty acids detected were anteiso-C (51.4 %), iso-C (5.3 %), C (12.1 %), iso-C (10.7 %) and anteiso-C (6.9 %). The DNA G+C content was determined to be 50.9 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-22460 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is FJAT-22460 (=DSM 100999=CCTCC AB 2015207).

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2016-11-01
2019-12-13
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References

  1. Ash C., Priest F. G., Collins M. D.. 1993; Molecular identification of rRNA group 3 bacilli (Ash, farrow, wallbanks and Collins) using a PCR probe test. proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek64:253–260[PubMed][CrossRef]
    [Google Scholar]
  2. Auch A. F., Von Jan M., Klenk H. P., Göker M.. 2010; Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci2:117–134 [CrossRef][PubMed]
    [Google Scholar]
  3. Buck J. D.. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol44:992–993[PubMed]
    [Google Scholar]
  4. Chen Y. G., Cui X. L., Pukall R., Li H. M., Yang Y. L., Xu L. H., Wen M. L., Peng Q., Jiang C. L.. 2007; Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. Int J Syst Evol Microbiol57:2327–2332 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  6. Cowan S. T., Steel K. J.. 1965; Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  7. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–789 [CrossRef]
    [Google Scholar]
  9. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  10. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A.. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol46:234–239 [CrossRef][PubMed]
    [Google Scholar]
  11. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kiese H. M., Lydiate D. J., Smith C. P., Ward J. M. et al. (editors) 1985; Genetic Manipulation of Streptomyces. a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  12. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules[M]. In Mammalian Protein Metabolismvol. 3 , pp.21–132 Edited by Munro H. N.. New York: Academic Press;[CrossRef]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Lee J. C., Kim C. J., Yoon K. H.. 2011; Paenibacillus telluris sp. nov., a novel phosphate-solubilizing bacterium isolated from soil. J Microbiol49:617–621 [CrossRef][PubMed]
    [Google Scholar]
  15. Lee J. S., Shin Y. K., Yoon J. H., Takeuchi M., Pyun Y. R., Park Y. H.. 2001; Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov., and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Evol Microbiol51:1491–1498 [CrossRef][PubMed]
    [Google Scholar]
  16. Lim J. M., Jeon C. O., Park D. J., Xu L. H., Jiang C. L., Kim C. J.. 2006; Paenibacillus xinjiangensis sp. nov., isolated from Xinjiang province in China. Int J Syst Evol Microbiol56:2579–2582 [CrossRef][PubMed]
    [Google Scholar]
  17. Meier-Kotloff J. P., Auch A. F., Klenk H. P., Göker M.. 2013; Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics14:60 [CrossRef][PubMed]
    [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high- performance liquid chromatography. Int J Syst Bacteriol39:159–167 [CrossRef]
    [Google Scholar]
  19. Minnikin D. E., Collins M. D., Goodfellow M.. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol47:87–95 [CrossRef]
    [Google Scholar]
  20. Murray R. G. E., Doetsch R. N., Robinow C. F.. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp.21–41 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington: American Society for Microbiology;
    [Google Scholar]
  21. Priest F. G.. 2009; Genus I. Paenibacillus. In Bergey's Manual of Systematic Bacteriologyvol. 3 pp.269–327 Edited by De Vos P., Garrity G., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B.. New York: Springer;
    [Google Scholar]
  22. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  23. Sasser M.. 1990; Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News Lett20:1–6
    [Google Scholar]
  24. Schumann P.. 2011; Peptidoglycan structure. In Taxonomy of Prokaryotes (Methods in Microbiology)vol. 38 pp101–129 Edited by Rainey F. A., Oren A.. Chennai: Academic Press;[CrossRef]
    [Google Scholar]
  25. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K.. 1997; Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov. Int J Syst Bacteriol47:299–306 [CrossRef][PubMed]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  27. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; MEGA6: molecular evolutionary genetics analysis 6.0. Mol Biol Evolution30:2725–2729[CrossRef]
    [Google Scholar]
  28. Tang Q. Y., Yang N., Wang J., Xie Y. Q., Ren B., Zhou Y. G., Gu M. Y., Mao J., Li W. J. et al. 2011; Paenibacillus algorifonticola sp. nov., isolated from a cold spring. Int J Syst Evol Microbiol61:2167–2172 [CrossRef][PubMed]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  30. Wang M., Yang M., Zhou G., Luo X., Zhang L., Tang Y., Fang C.. 2008; Paenibacillus tarimensis sp. nov., isolated from sand in Xinjiang, China. Int J Syst Evol Microbiol58:2081–2085 [CrossRef][PubMed]
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International committee on systematic bacteriology. Report of the adhoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
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