1887

Abstract

A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3b, grew at temperatures from 30 to 43 °C (optimum 37 °C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0–0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3b utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was used as an electron acceptor and converted to succinate. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate, nitrite, FeCl, Fe(III)-citrate, Fe(III)-EDTA, chromate, arsenate, selenate and DMSO were not used as terminal electron acceptors. The G+C content of the genomic DNA was 33.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family , order within the phylum . Strain 3b was most closely related to ‘' FatMR1 (92.2 % 16S rRNA gene sequence similarity), and was positioned approximately equidistantly between the genera , and . On the basis of phylogenetic, genetic, chemotaxonomic and physiological properties, strain 3b is proposed to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 3b (=DSM 100013=JCM 30645).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001375
2016-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4464.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001375&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and PSI-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: reevaluation of a unique biological group. Microbiol Rev 43:260–296[PubMed]
    [Google Scholar]
  3. Ben Aissa F., Postec A., Erauso G., Payri C., Pelletier B., Hamdi M., Ollivier B., Fardeau M. L. 2014; Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney. Int J Syst Evol Microbiol 64:1160–1165 [View Article][PubMed]
    [Google Scholar]
  4. Ben Aissa F., Postec A., Erauso G., Payri C., Pelletier B., Hamdi M., Fardeau M. L., Ollivier B. 2015; Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia. Extremophiles 19:183–188 [View Article][PubMed]
    [Google Scholar]
  5. Bes M., Merrouch M., Joseph M., Quéméneur M., Payri C., Pelletier B., Ollivier B., Fardeau M. L., Erauso G., Postec A. 2015; Acetoanaerobium pronyense sp. nov., an anaerobic mesophilic bacterium isolated from the Prony alkaline hydrothermal field, new caledonia. Int J Syst Evol Microbiol 65:2574–2580 [CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  7. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. J Microbiol Methods 4:33–36 [View Article]
    [Google Scholar]
  8. Edgar R. C. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  9. Fardeau M. L., Ollivier B., Patel B. K., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J. L. 1997; Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019 [View Article][PubMed]
    [Google Scholar]
  10. Hungate R. E. 1969; Chapter IV A roll tube method for cultivation of strict anaerobes. Method Microbiol 3:117–132 [CrossRef]
    [Google Scholar]
  11. Kelley D. S., Karson J. A., Früh-Green G. L., Yoerger D. R., Shank T. M., Butterfield D. A., Hayes J. M., Schrenk M. O., Olson E. J. et al. 2005; A serpentinite-hosted ecosystem: the lost city hydrothermal field. Science 307:1428–1434 [View Article][PubMed]
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and Deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Evol Microbiol 38:358–361 [View Article]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. Nucleic Acid Techniques. In Bacterial Systematics pp. 115–147 Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
    [Google Scholar]
  14. Launay J., Fontes J. C. 1985; Les sources thermales de Prony (Nouvelle-Calédonie) et leurs precipites chimiques. Exemple de formation de brucite primaire. Geologie De La France 1:83–100
    [Google Scholar]
  15. Mei N., Zergane N., Postec A., Erauso G., Ollier A., Payri C., Pelletier B., Fardeau M.-L., Ollivier B., Quéméneur M. 2014; Fermentative hydrogen production by a new alkaliphilic Clostridium sp. (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia. Int J Hydrogen Energy 39:19465–19473 [View Article]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bact 39:159–167 [View Article]
    [Google Scholar]
  17. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586[PubMed]
    [Google Scholar]
  18. Monnin C., Chavagnac V., Boulart C., Ménez B., Gérard M., Gérard E., Pisapia C., Quéméneur M., Erauso G. et al. 2014; Fluid chemistry of the low temperature hyperalkaline hydrothermal system of Prony bay (New Caledonia). Biogeosci 11:5687–5706 [View Article]
    [Google Scholar]
  19. Pikuta E. V., Itoh T., Krader P., Tang J., Whitman W. B., Hoover R. B. 2006; Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA. Int J Syst Evol Microbiol 56:2623–2629 [View Article][PubMed]
    [Google Scholar]
  20. Postec A., Quéméneur M., Bes M., Mei N., Benaïssa F., Payri C.m., Pelletier B., Monnin C., Guentas-Dombrowsky L. et al. 2015; Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period. Front Microbiol 6:857 [View Article][PubMed]
    [Google Scholar]
  21. Quéméneur M., Bes M., Postec A., Mei N., Hamelin J., Monnin C., Chavagnac V., Payri C., Pelletier B. et al. 2014; Spatial distribution of microbial communities in the shallow submarine alkaline hydrothermal field of the Prony Bay, new Caledonia. Environ Microbiol Rep 6:665–674 [View Article][PubMed]
    [Google Scholar]
  22. Quéméneur M., Palvadeau A., Postec A., Monnin C., Chavagnac V., Ollivier B., Erauso G. 2015; Endolithic microbial communities in carbonate precipitates from serpentinite-hosted hyperalkaline springs of the voltri massif (Ligurian Alps, Northern Italy). Environ Sci Pollut Res Int 22:13613–13624 [View Article][PubMed]
    [Google Scholar]
  23. Sasser M. 1990; Identification of Bacteria through Fatty Acid analysis. Meth Phytobacteriol199–204
    [Google Scholar]
  24. Takai K., Moser D. P., Onstott T. C., Spoelstra N., Pfiffner S. M., Dohnalkova A., Fredrickson J. K. 2001; Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. Int J Syst Evol Microbiol 51:1245–1256 [View Article][PubMed]
    [Google Scholar]
  25. Tamura K., Nei M., Kumar S. 2004; Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci 101:11030–11035 [View Article]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  27. Zhilina T. N., Detkova E. N., Rainey F. A., Osipov G. A., Lysenko A. M., Kostrikina N. A., Zavarzin G. A. 1998; Natronoincola histidinovorans gen. nov., sp. nov., a new alkaliphilic acetogenic anaerobe. Curr Microbiol 37:177–185 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001375
Loading
/content/journal/ijsem/10.1099/ijsem.0.001375
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error