1887

Abstract

A novel anaerobic, alkaliphilic, Gram-stain-positive, spore-forming bacterium was isolated from a carbonaceous hydrothermal chimney in Prony Bay, New Caledonia. This bacterium, designated strain 3b, grew at temperatures from 30 to 43 °C (optimum 37 °C) and at pH between 7.8 and 10.1 (optimum 9.5). Added NaCl was not required for growth (optimum 0–0.2 %, w/v), but was tolerated at up to 4 %. Yeast extract was required for growth. Strain 3b utilized crotonate, lactate and pyruvate, but not sugars. Crotonate was dismutated to acetate and butyrate. Lactate was disproportionated to acetate and propionate. Pyruvate was degraded to acetate plus trace amounts of hydrogen. Growth on lactate was improved by the addition of fumarate, which was used as an electron acceptor and converted to succinate. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate, nitrite, FeCl3, Fe(III)-citrate, Fe(III)-EDTA, chromate, arsenate, selenate and DMSO were not used as terminal electron acceptors. The G+C content of the genomic DNA was 33.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the isolate is a member of the family Clostridiaceae , order Clostridiales within the phylum Firmicutes . Strain 3b was most closely related to ‘ Alkaliphilus hydrothermalis ' FatMR1 (92.2 % 16S rRNA gene sequence similarity), and was positioned approximately equidistantly between the genera Alkaliphilus , Anaerovirgula and Natronincola . On the basis of phylogenetic, genetic, chemotaxonomic and physiological properties, strain 3b is proposed to represent a novel species of a new genus, for which the name Serpentinicella alkaliphila gen. nov., sp. nov. is proposed. The type strain of Serpentinicella alkaliphila is 3b (=DSM 100013=JCM 30645).

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2016-11-01
2019-10-16
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J..( 1997;). Gapped blast and PSI-blast: a new generation of protein database search programs. . Nucleic Acids Res25:3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S..( 1979;). Methanogens: reevaluation of a unique biological group. . Microbiol Rev43:260–296.[PubMed]
    [Google Scholar]
  3. Ben Aissa F., Postec A., Erauso G., Payri C., Pelletier B., Hamdi M., Ollivier B., Fardeau M. L..( 2014;). Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney. . Int J Syst Evol Microbiol64:1160–1165. [CrossRef][PubMed]
    [Google Scholar]
  4. Ben Aissa F., Postec A., Erauso G., Payri C., Pelletier B., Hamdi M., Fardeau M. L., Ollivier B..( 2015;). Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia. . Extremophiles19:183–188. [CrossRef][PubMed]
    [Google Scholar]
  5. Bes M., Merrouch M., Joseph M., Quéméneur M., Payri C., Pelletier B., Ollivier B., Fardeau M. L., Erauso G., Postec A..( 2015;). Acetoanaerobium pronyense sp. nov., an anaerobic mesophilic bacterium isolated from the Prony alkaline hydrothermal field, new caledonia. . Int J Syst Evol Microbiol65:2574–2580.[CrossRef]
    [Google Scholar]
  6. Cashion P., Holder-Franklin M. A., McCully J., Franklin M..( 1977;). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem81:461–466. [CrossRef][PubMed]
    [Google Scholar]
  7. Cord-Ruwisch R..( 1985;). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. . J Microbiol Methods4:33–36. [CrossRef]
    [Google Scholar]
  8. Edgar R. C..( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res32:1792–1797. [CrossRef][PubMed]
    [Google Scholar]
  9. Fardeau M. L., Ollivier B., Patel B. K., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J. L..( 1997;). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. . Int J Syst Bacteriol47:1013–1019. [CrossRef][PubMed]
    [Google Scholar]
  10. Hungate R. E..( 1969;). Chapter IV A roll tube method for cultivation of strict anaerobes. . Method Microbiol3:117–132.[CrossRef]
    [Google Scholar]
  11. Kelley D. S., Karson J. A., Früh-Green G. L., Yoerger D. R., Shank T. M., Butterfield D. A., Hayes J. M., Schrenk M. O., Olson E. J. et al.( 2005;). A serpentinite-hosted ecosystem: the lost city hydrothermal field. . Science307:1428–1434. [CrossRef][PubMed]
    [Google Scholar]
  12. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E..( 1988;). Fatty acids, antibiotic resistance, and Deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. . Int J Syst Evol Microbiol38:358–361. [CrossRef]
    [Google Scholar]
  13. Lane D. J..( 1991;). 16S/23S rRNA sequencing. Nucleic Acid Techniques. . In Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E., Goodfellow M.. New York:: Wiley;.
    [Google Scholar]
  14. Launay J., Fontes J. C..( 1985;). Les sources thermales de Prony (Nouvelle-Calédonie) et leurs precipites chimiques. Exemple de formation de brucite primaire. . Geologie De La France1:83–100.
    [Google Scholar]
  15. Mei N., Zergane N., Postec A., Erauso G., Ollier A., Payri C., Pelletier B., Fardeau M.-L., Ollivier B., Quéméneur M..( 2014;). Fermentative hydrogen production by a new alkaliphilic Clostridium sp. (strain PROH2) isolated from a shallow submarine hydrothermal chimney in Prony Bay, New Caledonia. . Int J Hydrogen Energy39:19465–19473. [CrossRef]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W..( 1989;). Precise measurement of the G+C content of Deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bact39:159–167. [CrossRef]
    [Google Scholar]
  17. Miller L. T..( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol16:584–586.[PubMed]
    [Google Scholar]
  18. Monnin C., Chavagnac V., Boulart C., Ménez B., Gérard M., Gérard E., Pisapia C., Quéméneur M., Erauso G. et al.( 2014;). Fluid chemistry of the low temperature hyperalkaline hydrothermal system of Prony bay (New Caledonia). . Biogeosci11:5687–5706. [CrossRef]
    [Google Scholar]
  19. Pikuta E. V., Itoh T., Krader P., Tang J., Whitman W. B., Hoover R. B..( 2006;). Anaerovirgula multivorans gen. nov., sp. nov., a novel spore-forming, alkaliphilic anaerobe isolated from Owens Lake, California, USA. . Int J Syst Evol Microbiol56:2623–2629. [CrossRef][PubMed]
    [Google Scholar]
  20. Postec A., Quéméneur M., Bes M., Mei N., Benaïssa F., Payri C.m., Pelletier B., Monnin C., Guentas-Dombrowsky L. et al.( 2015;). Microbial diversity in a submarine carbonate edifice from the serpentinizing hydrothermal system of the Prony Bay (New Caledonia) over a 6-year period. . Front Microbiol6:857. [CrossRef][PubMed]
    [Google Scholar]
  21. Quéméneur M., Bes M., Postec A., Mei N., Hamelin J., Monnin C., Chavagnac V., Payri C., Pelletier B. et al.( 2014;). Spatial distribution of microbial communities in the shallow submarine alkaline hydrothermal field of the Prony Bay, new Caledonia. . Environ Microbiol Rep6:665–674. [CrossRef][PubMed]
    [Google Scholar]
  22. Quéméneur M., Palvadeau A., Postec A., Monnin C., Chavagnac V., Ollivier B., Erauso G..( 2015;). Endolithic microbial communities in carbonate precipitates from serpentinite-hosted hyperalkaline springs of the voltri massif (Ligurian Alps, Northern Italy). . Environ Sci Pollut Res Int22:13613–13624. [CrossRef][PubMed]
    [Google Scholar]
  23. Sasser M..( 1990;). Identification of Bacteria through Fatty Acid analysis. . Meth Phytobacteriol, 199–204.
    [Google Scholar]
  24. Takai K., Moser D. P., Onstott T. C., Spoelstra N., Pfiffner S. M., Dohnalkova A., Fredrickson J. K..( 2001;). Alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep South African gold mine. . Int J Syst Evol Microbiol51:1245–1256. [CrossRef][PubMed]
    [Google Scholar]
  25. Tamura K., Nei M., Kumar S..( 2004;). Prospects for inferring very large phylogenies by using the neighbor-joining method. . Proc Natl Acad Sci101:11030–11035. [CrossRef]
    [Google Scholar]
  26. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  27. Zhilina T. N., Detkova E. N., Rainey F. A., Osipov G. A., Lysenko A. M., Kostrikina N. A., Zavarzin G. A..( 1998;). Natronoincola histidinovorans gen. nov., sp. nov., a new alkaliphilic acetogenic anaerobe. . Curr Microbiol37:177–185. [CrossRef][PubMed]
    [Google Scholar]
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