1887

Abstract

A Gram-stain-negative, rod-shaped, strictly aerobic, motile bacterial strain, designated TP162, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP162 was related to the genus Pseudoalteromonas and had highest 16S rRNA gene sequence similarities with the type strains Pseudoalteromonas shioyasakiensis SE3 (98.2 %), Pseudoalteromonas lipolytica LMEB 39 (97.7 %), P seudoalteromonas arabiensis k53 (97.4 %) and Pseudoalteromonas aliena KMM 3562 (97.2 %). The predominant cellular fatty acids were summed feature 3 (composed of iso-C1 5  : 0 2-OH and/or C1 6  : 1ω7c), C1 7  : 1ω8c and C1 6  : 0. The quinone system for strain TP162 comprised predominantly ubiquinone-8, and the polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and four unidentified lipids. The genomic DNA G+C content of strain TP162 was 46.7 mol%. Strain TP162 shared 28 % DNA–DNA relatedness with P . shioyasakiensis JCM 18891, 21 % with P. lipolytica JCM 15903, 35 % with P . arabiensis JCM 17292 and 18 % with P . aliena LMG 22059. Combined data from phenotypic, chemotaxonomic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain TP162 is a representative of a novel species of the genus Pseudoalteromonas , for which we propose the name Pseudoalteromonas profund i sp. nov. (type strain TP162=KACC 18554=CGMCC 1.15394).

Keyword(s): deep-sea , Pseudoalteromonas and seamount
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2016-11-01
2019-12-08
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J..( 1996;). Classification of bacteria isolated from a medieval wall painting. . J Biotechnol47:39–52. [CrossRef]
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D..( 1972;). Taxonomy of aerobic marine eubacteria. . J Bacteriol110:402–429.[PubMed]
    [Google Scholar]
  3. Bligh E. G., Dyer W. J..( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol37:911–917. [CrossRef][PubMed]
    [Google Scholar]
  4. Bowman J. P., McMeekin T. A..( 2005;). Genus XI. Pseudoalteromonas Gauthier, Gauthier and Christen 1995a, 759 vp. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn.,vol. 2B pp. 467–478. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York:: Springer;.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A..( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12:133–142. [CrossRef][PubMed]
    [Google Scholar]
  6. Dong X. Z., Cai M. Y..( (editors)) ( 2001;). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press (English translation);.
    [Google Scholar]
  7. Farmer J. J., Janda J. M., Brenner F. W., Cameron D. N., Birkhead K. M..( 2005;). Genus I. Vibrio Pacini 1854, 411AL366. . In Bergey’s Manual of Systematic Bacteriology the Proteobacteria, Part B, the Gammaproteo Bacteria, , 2nd edn.,vol. 2 pp. 494–546. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York:: Springer;.
  8. Gauthier G., Gauthier M., Christen R..( 1995;). Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. . Int J Syst Bacteriol45: 755–761. [CrossRef][PubMed]
    [Google Scholar]
  9. Huss V. A. R., Festl H., Schleifer K.-H..( 1983;). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol4:184–192. [CrossRef][PubMed]
    [Google Scholar]
  10. Ivanova E. P., Gorshkova N. M., Zhukova N. V., Lysenko A. M., Zelepuga E. A., Prokof'eva N. G., Mikhailov V. V., Nicolau D. V., Christen R..( 2004;). Characterization of Pseudoalteromonas distincta-like sea-water isolates and description of Pseudoalteromonas aliena sp. nov. . Int J Syst Evol Microbiol54:1431–1437. [CrossRef][PubMed]
    [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716–721. [CrossRef][PubMed]
    [Google Scholar]
  12. Kimura M..( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16:111–120. [CrossRef][PubMed]
    [Google Scholar]
  13. Leifson E..( 1963;). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol85:1183–1184.
    [Google Scholar]
  14. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F..( 2003;). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. . Extremophiles7:451–459. [CrossRef][PubMed]
    [Google Scholar]
  15. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5:109–118. [CrossRef][PubMed]
    [Google Scholar]
  16. Matsuyama H., Minami H., Kasahara H., Kato Y., Murayama M., Yumoto I..( 2013;). Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium. . Int J Syst Evol Microbiol63:1805–1809. [CrossRef][PubMed]
    [Google Scholar]
  17. Matsuyama H., Sawazaki K., Minami H., Kasahara H., Horikawa K., Yumoto I..( 2014;). Pseudoalteromonas shioyasakiensis sp. nov., a marine polysaccharide-producing bacterium. . Int J Syst Evol Microbiol64:101–106. [CrossRef][PubMed]
    [Google Scholar]
  18. Oh Y.-S., Park A.-R., Lee J.-K., Lim C.-S., Yoo J.-S., Roh D.-H..( 2011;). Pseudoalteromonas donghaensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol61:351–355. [CrossRef][PubMed]
    [Google Scholar]
  19. Park S., Jung Y.-T., Park D.-S., Yoon J.-H..( 2016;). Pseudoalteromonas aestuariivivens sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol66:2078–2083. [CrossRef][PubMed]
    [Google Scholar]
  20. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol4:406–425.[PubMed]
    [Google Scholar]
  21. Sambrook J., Russell D. W..( (editors)) ( 2001;). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory Press;.
    [Google Scholar]
  22. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, , Technical Note 101.. Newark, DE:: MIDI;.
    [Google Scholar]
  23. Stolz A., Busse H. J., Kämpfer P..( 2007;). Pseudomonas knackmussii sp. nov. . Int J Syst Evol Microbiol57:572–576. [CrossRef][PubMed]
    [Google Scholar]
  24. Süßmuth R., Eberspächer J., Haag R., Springer W..( 1987;). Biochemisch-Mikrobiologisches Praktikum. Stuttgart:: Georg Thieme Verlag;.
    [Google Scholar]
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  26. Thompson J. D., Plewniak F., Poch O..( 1999;). A comprehensive comparison of multiple sequence alignment programs. . Nucleic Acids Res27:2682–2690. [CrossRef][PubMed]
    [Google Scholar]
  27. Tindall B. J..( 1990a;). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol13:128–130. [CrossRef]
    [Google Scholar]
  28. Tindall B. J..( 1990b;). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett66:199–202. [CrossRef]
    [Google Scholar]
  29. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R..( 2007;). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Microbiology, , 3rd edn., pp. 330–393. Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R.. Washington, DC:: ASM Press;.
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Kandler O., Krichevsky M. I., Truper H. G., Murray R. G. E., Moore W. E. C., Grimont P. A. D. et al.( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Evol Microbiol37:463–464.[CrossRef]
    [Google Scholar]
  31. Xu X. W., Wu Y. H., Wang C. S., Gao X. H., Wang X. G., Wu M..( 2010;). Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary. . Int J Syst Evol Microbiol60:2176–2181. [CrossRef][PubMed]
    [Google Scholar]
  32. Zhang D. C., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J..( 2006;). Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. . Int J Syst Evol Microbiol56:2921–2925. [CrossRef][PubMed]
    [Google Scholar]
  33. Zhang D. C., Redzic M., Schinner F., Margesin R..( 2011;). Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. . Int J Syst Evol Microbiol61:2186–2190. [CrossRef][PubMed]
    [Google Scholar]
  34. Zhao C. H., Luo J. J., Gong T., Huang X. L., Ye D. Z., Luo Z. H..( 2014;). Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater. . Int J Syst Evol Microbiol64:444–448. [CrossRef][PubMed]
    [Google Scholar]
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