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Volume 66, Issue 11

sp. nov., isolated from a deep-sea seamount Free

Abstract

A Gram-stain-negative, rod-shaped, strictly aerobic, motile bacterial strain, designated TP162, was isolated from a seamount near the Yap Trench in the tropical western Pacific. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain TP162 was related to the genus and had highest 16S rRNA gene sequence similarities with the type strains SE3 (98.2 %), LMEB 39 (97.7 %), k53 (97.4 %) and KMM 3562 (97.2 %). The predominant cellular fatty acids were summed feature 3 (composed of iso-C 2-OH and/or C ω7), C ω8 and C . The quinone system for strain TP162 comprised predominantly ubiquinone-8, and the polar lipid profile contained phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and four unidentified lipids. The genomic DNA G+C content of strain TP162 was 46.7 mol%. Strain TP162 shared 28 % DNA–DNA relatedness with JCM 18891, 21 % with JCM 15903, 35 % with JCM 17292 and 18 % with LMG 22059. Combined data from phenotypic, chemotaxonomic, phylogenetic and DNA–DNA relatedness studies demonstrated that strain TP162 is a representative of a novel species of the genus , for which we propose the name sp. nov. (type strain TP162=KACC 18554=CGMCC 1.15394).

  • Received:
  • Accepted:
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Keyword(s): deep-sea , Pseudoalteromonas and seamount
© 2016 IUMS
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2016-11-01
2024-04-25
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References

  1. Altenburger P., Kämpfer P., Makristathis A., Lubitz W., Busse H.-J. 1996; Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47:39–52 [View Article]
     [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429[PubMed]
     [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [View Article][PubMed]
     [Google Scholar]
  4. Bowman J. P., McMeekin T. A. 2005; Genus XI. Pseudoalteromonas Gauthier, Gauthier and Christen 1995a, 759 vp. In Bergey's Manual of Systematic Bacteriology, 2nd edn. vol. 2B pp 467–478 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
     [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
     [Google Scholar]
  6. Dong X. Z., Cai M. Y. (editors) 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation);
     [Google Scholar]
  7. Farmer J. J., Janda J. M., Brenner F. W., Cameron D. N., Birkhead K. M. 2005; Genus I. Vibrio Pacini 1854, 411AL366. In Bergey’s Manual of Systematic Bacteriology the Proteobacteria, Part B, the Gammaproteo Bacteria, 2nd edn. vol. 2 pp 494–546 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
     [Google Scholar]
  8. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [View Article][PubMed]
     [Google Scholar]
  9. Huss V. A. R., Festl H., Schleifer K.-H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
     [Google Scholar]
  10. Ivanova E. P., Gorshkova N. M., Zhukova N. V., Lysenko A. M., Zelepuga E. A., Prokof'eva N. G., Mikhailov V. V., Nicolau D. V., Christen R. 2004; Characterization of Pseudoalteromonas distincta-like sea-water isolates and description of Pseudoalteromonas aliena sp. nov. Int J Syst Evol Microbiol 54:1431–1437 [View Article][PubMed]
     [Google Scholar]
  11. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  13. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
     [Google Scholar]
  14. Margesin R., Gander S., Zacke G., Gounot A. M., Schinner F. 2003; Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7:451–459 [View Article][PubMed]
     [Google Scholar]
  15. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
     [Google Scholar]
  16. Matsuyama H., Minami H., Kasahara H., Kato Y., Murayama M., Yumoto I. 2013; Pseudoalteromonas arabiensis sp. nov., a marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 63:1805–1809 [View Article][PubMed]
     [Google Scholar]
  17. Matsuyama H., Sawazaki K., Minami H., Kasahara H., Horikawa K., Yumoto I. 2014; Pseudoalteromonas shioyasakiensis sp. nov., a marine polysaccharide-producing bacterium. Int J Syst Evol Microbiol 64:101–106 [View Article][PubMed]
     [Google Scholar]
  18. Oh Y.-S., Park A.-R., Lee J.-K., Lim C.-S., Yoo J.-S., Roh D.-H. 2011; Pseudoalteromonas donghaensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 61:351–355 [View Article][PubMed]
     [Google Scholar]
  19. Park S., Jung Y.-T., Park D.-S., Yoon J.-H. 2016; Pseudoalteromonas aestuariivivens sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 66:2078–2083 [View Article][PubMed]
     [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  21. Sambrook J., Russell D. W. (editors) 2001 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
     [Google Scholar]
  22. Sasser M. 1990 Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, Technical Note 101. Newark, DE: MIDI;
     [Google Scholar]
  23. Stolz A., Busse H. J., Kämpfer P. 2007; Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57:572–576 [View Article][PubMed]
     [Google Scholar]
  24. Süßmuth R., Eberspächer J., Haag R., Springer W. 1987 Biochemisch-Mikrobiologisches Praktikum Stuttgart: Georg Thieme Verlag;
     [Google Scholar]
  25. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  26. Thompson J. D., Plewniak F., Poch O. 1999; A comprehensive comparison of multiple sequence alignment programs. Nucleic Acids Res 27:2682–2690 [View Article][PubMed]
     [Google Scholar]
  27. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
     [Google Scholar]
  28. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  29. Tindall B. J., Sikorski J., Smibert R. M., Kreig N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. pp 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G., Schmidt T. M., Snyder L. R. Washington, DC: ASM Press;
     [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Kandler O., Krichevsky M. I., Truper H. G., Murray R. G. E., Moore W. E. C., Grimont P. A. D. et al. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37:463–464 [CrossRef]
     [Google Scholar]
  31. Xu X. W., Wu Y. H., Wang C. S., Gao X. H., Wang X. G., Wu M. 2010; Pseudoalteromonas lipolytica sp. nov., isolated from the Yangtze River estuary. Int J Syst Evol Microbiol 60:2176–2181 [View Article][PubMed]
     [Google Scholar]
  32. Zhang D. C., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J. 2006; Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56:2921–2925 [View Article][PubMed]
     [Google Scholar]
  33. Zhang D. C., Redzic M., Schinner F., Margesin R. 2011; Glaciimonas immobilis gen. nov., sp. nov., a member of the family Oxalobacteraceae isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 61:2186–2190 [View Article][PubMed]
     [Google Scholar]
  34. Zhao C. H., Luo J. J., Gong T., Huang X. L., Ye D. Z., Luo Z. H. 2014; Pseudoalteromonas xiamenensis sp. nov., a marine bacterium isolated from coastal surface seawater. Int J Syst Evol Microbiol 64:444–448 [View Article][PubMed]
     [Google Scholar]
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Pseudoalteromonas profundi sp. nov., isolated from a deep-sea seamount
Int J Syst Evol Microbiol 66, 4416 (2016); https://doi.org/10.1099/ijsem.0.001366
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