1887

Abstract

A Gram-staining-negative, red-pigmented, motile, rod-shaped bacterial strain, designated as W14, was isolated from a hexachlorocyclohexane-contaminated dumpsite located in the northern part of India at Ummari Village, Lucknow. Phylogenetic analysis based on 16S rRNA gene sequence showed that the strain belongs to the genus with highest sequence similarity to DM9 (98.1 %). The 16S rRNA gene sequence similarity between strain W14 and members of other species of the genus ranged from 98.1 to 94.2 %. The DNA–DNA relatedness between strain W14 and DM9 was 33.7 % and with other closely related strains was found to be less than 20 %, confirming it to represent a novel species. The DNA G+C content of strain W14 was 51.3 mol%. Strain W14 was oxidase- and catalase-positive. The predominant cellular fatty acids were summed feature 4 (C iso I/anteiso B and C anteiso B/iso I), iso-C and iso-C 3-OH. The polar lipid profile of strain W14 consisted of phosphatidylethanolamine, diphosphatidylglycerol, aminolipid and glycolipid. On the basis of the results obtained from DNA–DNA hybridization, biochemical and physiological tests in this study, strain W14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W14 (=MCC 2932=DSM 100231=KCTC 42941).

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2016-11-01
2022-01-21
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