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Volume 66, Issue 11

sp. nov., a xylanolytic, methanol-utilizing bacterium isolated from the phyllosphere of bamboo () Free

Abstract

Strain BL24, isolated from bamboo phyllosphere collected in Coimbatore, India, was studied for taxonomic classification. Cells of the strain were aerobic, Gram-stain-positive, motile, catalase- and oxidase-positive rods and grew on media containing methanol. In 16S rRNA gene sequence analysis, strain BL24showed the highest sequence similarities with KACC 11473 (97.8 %) and SY01 (95.1 %). DNA–DNA hybridization with KACC 11473, phylogenetically the most closely related species, was 21.6 %; this value showed that strain BL24belonged to a different species. The cell-wall peptidoglycan was found to possess -diaminopimelic acid and the G+C content of genomic DNA was 52.1 mol %. It contained menaquinone (MK)-7 as the predominant respiratory quinone and the major cellular fatty acids are C, anteiso-C, iso-C, and anteiso-C. Based on the molecular and chemotaxonomic markers and physiological properties, strain BL24 (=NRRL B-51698=CCM 7577) is considered to represent a novel species of the genus , for which the name is proposed.

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Keyword(s): Bamboo , Paenibacillus methanolicus , phyllosphere , Pseudosasa japonica and Xylan
© 2016 IUMS
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2016-11-01
2024-04-20
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Paenibacillus methanolicus sp. nov., a xylanolytic, methanol-utilizing bacterium isolated from the phyllosphere of bamboo (Pseudosasa japonica)
Int J Syst Evol Microbiol 66, 4362 (2016); https://doi.org/10.1099/ijsem.0.001356
/content/journal/ijsem/10.1099/ijsem.0.001356
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