1887

Abstract

Strain BL24, isolated from bamboo phyllosphere collected in Coimbatore, India, was studied for taxonomic classification. Cells of the strain were aerobic, Gram-stain-positive, motile, catalase- and oxidase-positive rods and grew on media containing methanol. In 16S rRNA gene sequence analysis, strain BL24showed the highest sequence similarities with Paenibacillus phyllosphaeraeKACC 11473 (97.8 %) and Paenibacillus sacheonensisSY01 (95.1 %). DNA–DNA hybridization with P. phyllosphaerae KACC 11473, phylogenetically the most closely related species, was 21.6 %; this value showed that strain BL24belonged to a different species. The cell-wall peptidoglycan was found to possess meso-diaminopimelic acid and the G+C content of genomic DNA was 52.1 mol %. It contained menaquinone (MK)-7 as the predominant respiratory quinone and the major cellular fatty acids are C16 : 0, anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0. Based on the molecular and chemotaxonomic markers and physiological properties, strain BL24 (=NRRL B-51698=CCM 7577) is considered to represent a novel species of the genus Paenibacillus , for which the name Paenibacillus methanolicusis proposed.

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2016-11-01
2019-10-20
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