1887

Abstract

A strictly aerobic, Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 7Alg 4, was isolated from the green alga Cladophora stimpsonii. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain was affiliated to the family Flavobacteriaceae of the phylum Bacteroidetes , and was most closely related to the recognized species of the genera Lacinutrix and Flavirhabdus , with 16S rRNA gene sequence similarities of 95.1–98.1 and 97.0 %, respectively. Strain 7Alg 4 grew in the presence of 1–5 % NaCl and at 4–32 °C, and hydrolysed aesculin, gelatin, starch and Tween 80. The prevalent fatty acids were iso-C15 : 1 G, iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and C15 : 0. The polar lipid profile was characterized by the presence of phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The major respiratory quinone was MK-6. The DNA G+C content was 31.9 mol%. On the basis of the differences in 16S rRNA gene sequences, chemotaxonomic and phenotypic characteristics, it is suggested that strain 7Alg 4 represents a novel species of the genus Lacinutrix , for which the name Lacinutrix cladophorae sp. nov. is proposed. The type strain is 7Alg 4 (=KCTC 23036=KMM 6381). Reclassification of Flavirhabdus iliipiscaria as Lacinutrix iliipiscaria comb. nov. and an emend of the genus Lacinutrix are also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001353
2016-11-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/11/4339.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001353&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B..for Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol52:1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Bligh E. G., Dyer W. J..( 1959;). A rapid method of total lipid extraction and purification. . Can J Biochem Physiol37:911–917. [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P., Nichols D. S..( 2005;). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. . Int J Syst Evol Microbiol55:1471–1486. [CrossRef][PubMed]
    [Google Scholar]
  4. Chen W. P., Kuo T. T..( 1993;). A simple and rapid method for the preparation of gram-negative bacterial genomic DNA. . Nucleic Acids Res21:2260. [CrossRef][PubMed]
    [Google Scholar]
  5. Collins M. D., Shah H. N..( 1984;). Fatty acid, menaquinone and polar lipid composition of Rothia dentocariosa. . Arch Microbiol137:247–249. [CrossRef]
    [Google Scholar]
  6. Fautz E., Reichenbach H..( 1980;). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett8:87–91. [CrossRef]
    [Google Scholar]
  7. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution39:783–791. [CrossRef]
    [Google Scholar]
  8. Felsenstein J..( 1993;). phylip (phylogenetic inference package) version 3.5.1. . Department of Genetics, University of Washington, Seattle.
  9. Huang Z., Li G., Lai Q., Gu L., Shao Z..( 2016;). Lacinutrix gracilariae sp. nov., isolated from the surface of a marine red alga Gracilaria sp. . Int J Syst Evol Microbiol66:587–591. [CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol62:716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K.-I..( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol19:161–207.[CrossRef]
    [Google Scholar]
  12. Lee Y. M., Hwang C. Y., Lee I., Jung Y.-J., Cho Y., Baek K., Hong S. G., Kim J.-H., Chun J., Lee H. K..( 2014;). Lacinutrix jangbogonensis sp. nov., a psychrophilic bacterium isolated from Antarctic marine sediment and emended description of the genus Lacinutrix. . Antonie Van Leeuwenhoek106:527–533. [CrossRef][PubMed]
    [Google Scholar]
  13. Marmur J..( 1961;). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol3:208–218. [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5:109–118. [CrossRef][PubMed]
    [Google Scholar]
  15. Nedashkovskaya O. I., Kwon K. K., Yang S. H., Lee H. S., Chung K. H., Kim S. J..( 2008;). Lacinutrix algicola sp. nov. and Lacinutrix mariniflava sp. nov., two novel marine alga-associated bacteria and emended description of the genus Lacinutrix. . Int J Syst Evol Microbiol58:2694–2698. [CrossRef][PubMed]
    [Google Scholar]
  16. Nedashkovskaya O. I., Kukhlevskiy A. D., Zhukova N. V., Kim S.-J., Rhee S.-K..( 2013;). Litorimonas cladophorae sp. nov., a new alphaproteobacterium isolated from the Pacific green alga Cladophora stimpsonii, and emended descriptions of the genus Litorimonas and Litorimonas taeaensis. . Antonie Van Leeuwenhoek103:1263–1269. [CrossRef][PubMed]
    [Google Scholar]
  17. Nedashkovskaya O. I., Kukhlevskiy A. D., Zhukova N. V., Kim S.-J., Rhee S.-K., Mikhailov V. V..( 2015;). Winogradskyella litoriviva sp. nov., isolated from coastal seawater. . Int J Syst Evol Microbiol65:3652–3657. [CrossRef][PubMed]
    [Google Scholar]
  18. Park S., Park J. M., Jung Y. T., Kang C. H., Yoon J. H..( 2015;). Lacinutrix undariae sp. nov., isolated from a brown algae reservoir. . Int J Syst Evol Microbiol65:2696–2701. [CrossRef][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol4:406–425.[PubMed]
    [Google Scholar]
  20. Sasser M..( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl20:1–6.
    [Google Scholar]
  21. Shakeela Q., Shehzad A., Zhang Y., Tang K., Zhang X. H..( 2015;). Flavirhabdus iliipiscaria gen. nov., sp. nov., isolated from intestine of flounder (Paralichthys olivaceus) and emended descriptions of the genera Flavivirga, Algibacter, Bizionia and Formosa. . Int J Syst Evol Microbiol65:1347–1353. [CrossRef][PubMed]
    [Google Scholar]
  22. Srinivas T. N. R., Prasad S., Manasa P., Sailaja B., Begum Z., Shivaji S..( 2013;). Lacinutrix himadriensis sp. nov., a psychrophilic bacterium isolated from a marine sediment, and emended description of the genus Lacinutrix. . Int J Syst Evol Microbiol63:729–734. [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Ebers J..( 2006;). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today33:152–155.
    [Google Scholar]
  24. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S..( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol30:2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  25. Thompson J. D., Higgins D. G., Gibson T. J..( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res22:4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  26. Yi H., Cho J. C., Chun J..( 2012;). Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix. . Int J Syst Evol Microbiol62:1061–1068. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001353
Loading
/content/journal/ijsem/10.1099/ijsem.0.001353
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error