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Abstract

A Gram-positive-staining, endospore-forming, facultatively anaerobic, lactic-acid-producing bacterium, strain GD201205, was isolated from spoiled jelly in China. Strain GD201205 fermented glucose, fructose, mannose, sucrose, raffinose and turanose, but negative for nitrate reduction, catalase and oxidase. The predominant fatty acids of the strain were anteiso-C17 : 0 and anteiso-C15 : 0. Whole-cell hydrolysates contained glycine and alanine with meso-iaminopimelic acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, glycolipid 1 and glycolipid 2. The DNA G+C content of strain GD201205 was 48.7 mol%. 16S rRNA gene sequence analysis indicated that the strain belonged to the genus Sporolactobacillus and was most closely related to Sporolactobacillus vineae KCTC 5376 and Sporolactobacillus putidus JCM 15325 with 16S rRNA gene sequence similarities of 97.5 and 96.9 %, respectively. Levels of DNA–DNA relatedness between strain GD201205 and Sporolactobacillus vineae KCTC 5376and Sporolactobacillus putidus JCM 15325 were 29.2 and 47.6 %, respectively. Phylogenetic analysis based on the 16S rRNA gene and gyrB gene revealed that strain GD201205 was clearly distinct from all related species of the genus Sporolactobacillus . On the basis of the phylogenetic, chemotaxonomic and phenotypic evidence given in this study, strain GD201205 should be classified as a representative of a novel species of the genus Sporolactobacillus for which the name Sporolactobacillus pectinivorans is proposed. The type strain is GD201205 (=CICC 23867=KCTC 15488).

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2016-11-01
2019-10-23
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