In this study, we identified a novel Gram-stain-negative, aerobic, motile, and rod-shaped bacterium, strain KX 20T, isolated from the deep seawater in Okinawa Trough, northwestern Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KX 20T was related to members of the genus Pseudomonas and shares the highest sequence identities with Pseudomonas aestusnigri CECT 8317T (99.4 %) and Pseudomonas pachastrellae JCM 12285T (98.5 %). The 16S rRNA gene sequence identities between strain KX 20T and other members of the genus Pseudomonaswere below 96.6 %. The gyrB and rpoD genes of strain KX 20T shared 82.0 to 89.3 % sequence identity with the gyrB and rpoD genes of the closest phylogenetic neighbours of KX 20T. The predominant cellular fatty acids of strain KX 20T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (29.2 %), C16 : 0 (24.5 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) (21.5 %) and C12 : 0 (8.2 %). The major polar lipids of strain KX 20T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipids. The genomic DNA G+C content of strain KX 20T was 62.9 mol%. On the basis of phylogenetic analysis and phenotypic characteristics, a novel species, Pseudomonas oceani sp. nov. is proposed. The type strain is KX 20T (=CGMCC 1.15195T=DSM 100277T).
AmoozegarM. A.,
ShahinpeiA.,
SepahyA. A.,
Makhdoumi-KakhkiA.,
SeyedmahdiS. S.,
SchumannP.,
VentosaA.2014; Pseudomonas salegens sp. nov., a halophilic member of the genus Pseudomonas isolated from a wetland. Int J Syst Evol Microbiol 64:3565–3570 [View Article][PubMed]
BernardetJ. F.,
NakagawaY.,
HolmesB.2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
DoetschR. N.1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology , pp. 21–33 Edited by
GerdhartP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
Washington, DC: American Society for Microbiology;
FrankJ. A.,
ReichC. I.,
SharmaS.,
WeisbaumJ. S.,
WilsonB. A.,
OlsenG. J.2008; Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Appl Environ Microbiol 74:2461–2470 [View Article][PubMed]
KimK. H.,
RohS. W.,
ChangH. W.,
NamY. D.,
YoonJ. H.,
JeonC. O.,
OhH. M.,
BaeJ. W.2009; Pseudomonas sabulinigri sp. nov., isolated from black beach sand. Int J Syst Evol Microbiol 59:38–41 [View Article][PubMed]
KimuraM.1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
KobayashiT.,
KoideO.,
MoriK.,
ShimamuraS.,
MatsuuraT.,
MiuraT.,
TakakiY.,
MoronoY.,
NunouraT. et al.2008; Phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off Shimokita Peninsula. Extremophiles 12:519–527 [View Article][PubMed]
LiuC.,
ShaoZ.2005; Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55:1181–1186 [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise masurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. Microbiol Methods 2:233–241 [View Article]
MooreE. R. B.,
ArnscheidtA.,
KrügerA.,
StrömplC.,
MauM.1999; Simplified protocols for the preparation of genomic DNA from bacterial cultures. In Molecular Microbial Ecology Manual 1.6.1 , pp. 1–15 Edited by
AkkermansA. D. L.,
van ElsasJ. D.,
de BruijnF. J.
Kluwer: Dordrecht;
MuletM.,
GomilaM.,
ScottaC.,
SánchezD.,
LalucatJ.,
García-ValdésE.2012b; Concordance between whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and multilocus sequence analysis approaches in species discrimination within the genus Pseudomonas
. Syst Appl Microbiol 35:455–464 [View Article]
PalleroniN. J.1984; Genus I. Pseudomonas Migula 1984, 237AL
. In Berger’s Manual of Systematic Bacterioologyvol. 1 , pp. 141–199 Edited by
KriegN. R.,
HoltJ. G.
Baltimore: Williams & Wilkins;
PalleroniN. J.2005; Pseudomonas. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 2 , pp. 323–379 Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.
New York, NY: Springer;
TindallB. J.,
SikorskiJ.,
SmibertR. M.,
Krieg.N. R.2007; Phenotypic characterization and the principles of comparative systematic. In Methods for General and Molecular Microbiology, 3rd edn. pp. 330–393 Edited by
ReddyC. A.,
BeveridgeT. J.,
BreznakJ. A.,
MarzlufG. A.,
SchmidtT. M.,
SnyderL. R.
Washington, DC: American Society for Microbiology;
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM.I,
MooreW. E. C.,
MurrayR. G. E. et al.1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematic. Int J Syst Bacteriol 37:463–564[CrossRef]