1887

International Journal of Systematic and Evolutionary Microbiology

Volume 66, Issue 10

Description of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales Free

Abstract

A Gram-stain-negative, facultatively anaerobic, moderately halophilic, filamentous, non-motile bacterium, designated FA102, was isolated from marine sediment from the coast of Weihai, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain FA102 formed a distinct evolutionary lineage within the family Marinifilaceae and its closest relative was Marinifilum fragile JCM 15579 (93.2 % sequence similarity). The DNA G+C content of the novel strain was 36.5 mol%. The predominant cellular fatty acids and respiratory quinone were iso-C15 : 0 and iso-C15 : 0 3-OH, and MK-7, respectively. On the basis of the phylogenetic, phenotypic and physiological data, strain FA102 represents a novel genus and species, for which the name Ancylomarina subtilis gen. nov., sp. nov. is proposed. The type strain of Ancylomarina subtilis is FA102 (=KCTC 42257=DSM 28825=CICC 10902). Furthermore, a new order named Marinilabiliales is proposed to accommodate three families previously classified in the order Bacteroidales . Marinilabiliales ord. nov. encompasses the families Marinilabiliaceae , Prolixibacteraceae and Marinifilaceae.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Ancylomarina subtilis gen. nov., sp. nov. , Bacterioidia , Marinifilaceae and Marinilabiliales
© 2016 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001342
2016-10-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4243.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001342&mimeType=html&fmt=ahah

References

  1. Abe K., Ueki A., Ohtaki Y., Kaku N., Watanabe K., Ueki K..( 2012;). Anaerocella delicata gen. nov., sp. nov., a strictly anaerobic bacterium in the phylum Bacteroidetes isolated from a methanogenic reactor of cattle farms. . J Gen Appl Microbiol 58: 405––412. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bouanane-Darenfed A., Ben Hania W., Cayol J.-L., Ollivier B., Fardeau M.-L..( 2015;). Reclassification of Acetomicrobium faecale as Caldicoprobacter faecalis comb. nov. . Int J Syst Evol Microbiol 65: 3286––3288. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P..( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50: 1861––1868. [CrossRef] [PubMed]
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y..( 2001;). Determination of biochemical characteristics. . In Manual for the Systematic Identification of General Bacteria, pp. 370––398. Edited by Dong X.-Z., Cai M.-Y.. Beijing:: Science Press (in Chinese);.
     [Google Scholar]
  5. Downes J., Dewhirst F. E., Tanner A. C. R., Wade W. G..( 2013;). Description of Alloprevotella rava gen. nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al. 1994 as Alloprevotella tannerae gen. nov., comb. nov. . Int J Syst Evol Microbiol 63: 1214––1218. [CrossRef] [PubMed]
    [Google Scholar]
  6. Du Z. J., Wang Y., Dunlap C., Rooney A. P., Chen G. J..( 2014;). Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. . Int J Syst Evol Microbiol 64: 1690––1696. [CrossRef] [PubMed]
    [Google Scholar]
  7. Gao Z. M., Liu X., Zhang X. Y., Ruan L. W..( 2013;). Thermophagus xiamenensis gen. nov., sp. nov., a moderately thermophilic and strictly anaerobic bacterium isolated from hot spring sediment. . Int J Syst Evol Microbiol 63: 109––113. [CrossRef] [PubMed]
    [Google Scholar]
  8. Hania W. B., Bouanane-Darenfed A., Fardeau M.-L., Cayol J.-L., Ollivier B..( 2016;). Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium. . Int J Syst Evol Microbiol 66: 1506––1509. [CrossRef]
    [Google Scholar]
  9. Hardham J. M., King K. W., Dreier K., Wong J., Strietzel C., Eversole R. R., Sfintescu C., Evans R. T..( 2008;). Transfer of Bacteroides splanchnicus to Odoribacter gen. nov. as Odoribacter splanchnicus comb. nov., and description of Odoribacter denticanis sp. nov., isolated from the crevicular spaces of canine periodontitis patients. . Int J Syst Evol Microbiol 58: 103––109. [CrossRef] [PubMed]
    [Google Scholar]
  10. Holmes D. E., Nevin K. P., Woodard T. L., Peacock A. D., Lovley D. R..( 2007;). Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. . Int J Syst Evol Microbiol 57: 701––707. [CrossRef] [PubMed]
    [Google Scholar]
  11. Huang X. F., Liu Y. J., Dong J. D., Qu L. Y., Zhang Y. Y., Wang F. Z., Tian X. P., Zhang S..( 2014;). Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. . Int J Syst Evol Microbiol 64: 875––881. [CrossRef] [PubMed]
    [Google Scholar]
  12. Iino T., Mori K., Itoh T., Kudo T., Suzuki K., Ohkuma M..( 2014;). Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov. . Int J Syst Evol Microbiol 64: 3660––3667. [CrossRef] [PubMed]
    [Google Scholar]
  13. Irgens R. L..( 1977;). Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. . Int J Syst Bacteriol 27: 38––43. [CrossRef]
    [Google Scholar]
  14. Jabari L., Gannoun H., Cayol J. L., Hedi A., Sakamoto M., Falsen E., Ohkuma M., Hamdi M., Fauque G. et al.( 2012;). Macellibacteroides fermentans gen. nov., sp. nov., a member of the family Porphyromonadaceae isolated from an upflow anaerobic filter treating abattoir wastewaters. . Int J Syst Evol Microbiol 62: 2522––2527. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716––721. [CrossRef] [PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K..( 1987;). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19: 161––207.[CrossRef]
    [Google Scholar]
  17. Krieg N. R..( 2011;). Class I. Bacteroidia class. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 4 pp. 25––104 . Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B.. New York:: Springer;.
     [Google Scholar]
  18. Liu Q.-Q., Wang Y., Li J., Du Z.-J., Chen G.-J..( 2014a;). Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. . Int J Syst Evol Microbiol 64: 2204––2209. [CrossRef] [PubMed]
    [Google Scholar]
  19. Liu Q.-Q., Li X.-L., Rooney A. P., Du Z.-J., Chen G.-J..( 2014b;). Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae. . Int J Syst Evol Microbiol 64: 3473––3477. [CrossRef] [PubMed]
    [Google Scholar]
  20. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al.( 2004;). arb: a software environment for sequence data. . Nucleic Acids Res 32: 1363. [CrossRef] [PubMed]
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159––167. [CrossRef]
    [Google Scholar]
  22. Miyazaki M., Koide O., Kobayashi T., Mori K., Shimamura S., Nunoura T., Imachi H., Inagaki F., Nagahama T. et al.( 2012;). Geofilum rubicundum gen. nov., sp. nov., isolated from deep subseafloor sediment. . Int J Syst Evol Microbiol 62: 1075––1080. [CrossRef] [PubMed]
    [Google Scholar]
  23. Moore L. V. H., Moore W. E. C..( 1994;). Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., anaerobic Gram-negative bacilli from the human gingival crevice. . Int J Syst Bacteriol 44: 187––192. [CrossRef] [PubMed]
    [Google Scholar]
  24. Morotomi M., Nagai F., Sakon H., Tanaka R..( 2009;). Paraprevotella clara gen. nov., sp. nov. and Paraprevotella xylaniphila sp. nov., members of the family ‘Prevotellaceae’ isolated from human faeces. . Int J Syst Evol Microbiol 59: 1895––1900. [CrossRef] [PubMed]
    [Google Scholar]
  25. Na H., Kim S., Moon E. Y., Chun J..( 2009;). Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 59: 2241––2246. [CrossRef] [PubMed]
    [Google Scholar]
  26. Nelson M. C., Bomar L., Maltz M., Graf J..( 2015;). Mucinivorans hirudinis gen. nov., sp. nov., an anaerobic, mucin-degrading bacterium isolated from the digestive tract of the medicinal leech Hirudo verbana. . Int J Syst Evol Microbiol 65: 990––995. [CrossRef] [PubMed]
    [Google Scholar]
  27. Pruesse E., Peplies J., Glöckner F. O..( 2012;). SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28: 1823––1829. [CrossRef] [PubMed]
    [Google Scholar]
  28. Qu L., Zhu F., Hong X., Gao W., Chen J., Sun X..( 2011;). Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes. . Int J Syst Evol Microbiol 61: 2885––2889.[CrossRef]
    [Google Scholar]
  29. Ruvira M. A., Lucena T., Pujalte M. J., Arahal D. R., Macián M. C..( 2013;). Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal mediterranean sea water and emended description of the genus Marinifilum Na et al. 2009. . Syst Appl Microbiol 36: 155––159. [CrossRef] [PubMed]
    [Google Scholar]
  30. Sakamoto M., Benno Y..( 2006;). Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov. . Int J Syst Evol Microbiol 56: 1599––1605. [CrossRef] [PubMed]
    [Google Scholar]
  31. Sakamoto M., Takagaki A., Matsumoto K., Kato Y., Goto K., Benno Y..( 2009;). Butyricimonas synergistica gen. nov., sp. nov. and Butyricimonas virosa sp. nov., butyric acid-producing bacteria in the family ‘Porphyromonadaceae’ isolated from rat faeces. . Int J Syst Evol Microbiol 59: 1748––1753. [CrossRef] [PubMed]
    [Google Scholar]
  32. Sánchez-Andrea I., Sanz J. L., Stams A. J. M..( 2014;). Microbacter margulisiae gen. nov., sp. nov., a propionigenic bacterium isolated from sediments of an acid rock drainage pond. . Int J Syst Evol Microbiol 64: 3936––3942. [CrossRef] [PubMed]
    [Google Scholar]
  33. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  34. Shkoporov A. N., Khokhlova E. V., Chaplin A. V., Kafarskaia L. I., Nikolin A. A., Polyakov V. Y., Shcherbakova V. A., Chernaia Z. A., Efimov B. A..( 2013;). Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces. . Int J Syst Evol Microbiol 63: 4181––4188. [CrossRef] [PubMed]
    [Google Scholar]
  35. Smibert R. M., Krieg N. R..( 1994;). Phenotypic characterization. . In Methods for General And Molecular Bacteriology , pp. 607––654 . Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  36. Sorokin D. Y., Panteleeva A. N., Tourova T. P., Kaparullina E. N., Muyzer G..( 2011;). Natronoflexus pectinivorans gen. nov. sp. nov., an obligately anaerobic and alkaliphilic fermentative member of Bacteroidetes from soda lakes. . Extremophiles 15: 691––696. [CrossRef] [PubMed]
    [Google Scholar]
  37. Su X.-L., Tian Q., Zhang J., Yuan X.-Z., Shi X.-S., Guo R.-B., Qiu Y.-L..( 2014;). Acetobacteroides hydrogenigenes gen. nov., sp. nov., an anaerobic hydrogen-producing bacterium in the family Rikenellaceae isolated from a reed swamp. . Int J Syst Evol Microbiol 64: 2986––2991. [CrossRef] [PubMed]
    [Google Scholar]
  38. Takai K., Abe M., Miyazaki M., Koide O., Nunoura T., Imachi H., Inagaki F., Kobayashi T..( 2013;). Sunxiuqinia faeciviva sp. nov., a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment. . Int J Syst Evol Microbiol 57: 701––707.
     [Google Scholar]
  39. Wagener K., Drillich M., Baumgardt S., Kämpfer P., Busse H. J., Ehling-Schulz M..( 2014;). Falsiporphyromonas endometrii gen. nov., sp. nov., isolated from the post-partum bovine uterus, and emended description of the genus Porphyromonas Shah and Collins 1988. . Int J Syst Evol Microbiol 64: 642––649. [CrossRef] [PubMed]
    [Google Scholar]
  40. Wang Z.-J., Xie Z.-H., Wang C., Du Z.-J., Chen G.-J..( 2014;). Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment. . Antonie Van Leeuwenhoek 106: 615––621. [CrossRef] [PubMed]
    [Google Scholar]
  41. Wu W.-J., Liu Q.-Q., Chen G.-J., Du Z.-J..( 2015;). Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment. . Int J Syst Evol Microbiol 65: 2260––2264. [CrossRef] [PubMed]
    [Google Scholar]
  42. Yang S.-H., Seo H.-S., Woo J.-H., Oh H.-M., Jang H., Lee J.-H., Kim S.-J., Kwon K. K..( 2014;). Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga fermentans as Saccharicrinis fermentans gen. nov., comb. nov. . Int J Syst Evol Microbiol 64: 1351––1358. [CrossRef] [PubMed]
    [Google Scholar]
  43. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R..( 2008;). The all-species living tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31: 241––250. [CrossRef] [PubMed]
    [Google Scholar]
  44. Zhao B., Chen S..( 2012;). Alkalitalea saponilacus gen. nov., sp. nov., an obligately anaerobic, alkaliphilic, xylanolytic bacterium from a meromictic soda lake. . Int J Syst Evol Microbiol 62: 2618––2623. [CrossRef] [PubMed]
    [Google Scholar]
  45. Zhao C., Gao Z., Qin Q., Ruan L..( 2012;). Mangroviflexus xiamenensis gen. nov., sp. nov., a member of the family Marinilabiliaceae isolated from mangrove sediment. . Int J Syst Evol Microbiol 62: 1819––1824. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001342
Loading
Description of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales
Int J Syst Evol Microbiol 66, 4243 (2016); https://doi.org/10.1099/ijsem.0.001342
/content/journal/ijsem/10.1099/ijsem.0.001342
/content/journal/ijsem/10.1099/ijsem.0.001342
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error