1887

Abstract

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, HPTF-2, was isolated from a tidal flat in the Yellow Sea in South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain HPTF-2 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain HPTF-2 fell within the clade comprising the type strains of species of the genus Maribacter . Strain HPTF-2 exhibited 16S rRNA gene sequence similarity values of 97.1–98.0 % to the type strains of Maribacter stanieri , M. orientalis, M. arcticus, M. dokdonensis , M. confluentis, M. aquivivus, M. sedimenticola and M. caenipelagi , and of 95.1–96.7 % to the type strains of the other species of the genus Maribacter . Strain HPTF-2 contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the major fatty acids. The major polar lipids of strain HPTF-2 were phosphatidylethanolamine and two unidentified lipids. Mean DNA–DNA relatedness values of strain HPTF-2 with the type strains of the nine phylogenetically related species of the genus Maribacter were 10–26 %. The DNA G+C content of strain HPTF-2 was 38.4 mol%. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain HPTF-2 is separated from other species of the genus Maribacter . On the basis of the data presented, strain HPTF-2 represents a novel species of the genus Maribacter , for which the name Maribacter litorisediminis sp. nov. is proposed. The type strain is HPTF-2 (=KCTC 52251=NBRC 112297).

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2016-10-01
2019-12-07
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