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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 66, Issue 10

sp. nov., an actinomycete isolated from phylloplane of pine ( L.) needle-like leaves Free

Abstract

A novel siderophore-producing actinomycete, designated PL19, was isolated from the Scots-pine needle-like leaves collected from TNAU campus, Coimbatore, India. The isolate was chemoorganotrophic in nutrition and able to grow at 30 °C, and the optimum pH and NaCl facilitated the growth pH 6–11 and 0–8 % (w/v), respectively. The cells are filamentous and the mycelia formed are basically of wide and intricately branched substrate mycelium from which aerial mycelia arises, later gets differentiated into spores that are warty and arranged spirally. The 16S rRNA gene of strain PL19 was sequenced and was highly similar to the type strains of species of the genus , including RC1831 (98.8 % pairwise similarity), GIMN4.003 (98.2 %), SCSIO 01248 (98.0 %), R97 (97.9 %), C60 (97.8 %) and NBRC 14749 (97.2 %), all of which were subjected to taxonomical characterization using a polyphasic approach. The strains showed unique carbon utilization patterns, and it possesses iso-C anteiso-C and anteiso-C as a major cellular fatty acids. The cell-wall was dominated with -type diaminopimelic acid, and the menaquinone type was MK-9(H, H). These chemotaxonomic evidences placed strain PL19 within the genus . The determination of G+C ratio (69.5 mol%) and DNA–DNA hybridization values (13.4–31.8 % with the phylogenetically related species) helped in further hierarchical classification of strain PL19. Based on morphological, physiological and chemotaxonomic data as well as DNA–DNA hybridization values, strain PL19 could be distinguished from the evolutionarily closest species currently available. All these collective data show that strain PL19 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is PL19 (=NRRL B-24728=ICMP 17783).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): needle leaves , phylloplane , Pinus sylvestris , siderophore and Streptomycespini
© 2016 IUMS
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Streptomyces pini sp. nov., an actinomycete isolated from phylloplane of pine (Pinus sylvestris L.) needle-like leaves
Int J Syst Evol Microbiol 66, 4204 (2016); https://doi.org/10.1099/ijsem.0.001336
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