A Gram-stain-positive, facultatively anaerobic, non-spore-forming, non-flagellated and rod-shaped or ovoid bacterial strain, GHD-1T, was isolated from a tidal flat sediment on the Yellow Sea, South Korea. It grew optimally at 30 °C, at pH 6.5–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain GHD-1T fell within the cluster comprising the type strains of species of the genus Demequina. Strain GHD-1T exhibited 16S rRNA gene sequence similarity values of 97.0–98.8 % to the type strains of Demequinaglobuliformis, D. salsinemoris, D. flava, D. sediminicola and D. activiva, and of 95.5–96.9 % to the type strains of the other species of the genus Demequina. The peptidoglycan type of strain GHD-1T is A4β based on l-Orn−l-Ser−l-Asp. It contained DMK-9(H4) and DMK-8(H4) as the major menaquinones and anteiso-C15 : 0, C16 : 0, iso-C16 : 0 and anteiso-C17 : 0 as the major fatty acids. The major polar lipids were phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified glycolipid. The DNA G+C content of strain GHD-1T was 68.7 mol%, and its DNA–DNA relatedness values with the type strains of five phylogenetically related species of the genus Demequina were 13–29 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHD-1T is separated from other recognized species of the genus Demequina. On the basis of the data presented, strain GHD-1T is considered to represent a novel species of the genus Demequina, for which the name Demequina litorisediminis sp. nov. is proposed. The type strain is GHD-1T (=KCTC 52260T=NBRC 112299T).
BarrowG. I.,
FelthamR. K. A.1993Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [CrossRef]
BaumannP.,
BaumannL.1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes
. In The Prokaryotes , pp. 1302–1331 Edited by
StarrM. P.,
StolpH.,
TrüperH. G.,
BalowsA.,
SchlegelH. G.
Berlin: Springer;
EmbleyT. M.,
WaitR.1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methodsin Prokaryotic Systematics , pp. 121–161 Edited by
GoodfellowM.,
O’DonnellA. G.
Chichester: John Wiley & Sons;
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
FinsterK. W.,
HerbertR. A.,
KjeldsenK. U.,
SchumannP.,
LomsteinB. A.2009; Demequina lutea sp. nov., isolated from a high Arctic permafrost soil. Int J Syst Evol 59:649–653 [View Article]
HamadaM.,
TamuraT.,
YamamuraH.,
SuzukiK.,
HayakawaM.2012; Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove. Int J Syst Evol Microbiol 62:1731–1735 [View Article][PubMed]
MatsumotoA.,
NakaiK.,
MorisakiK.,
OmuraS.,
TakahashiY.2010; Demequina salsinemoris sp. nov., isolated on agar media supplemented with ascorbic acid or rutin. Int J Syst Evol Microbiol 60:1206–1209 [View Article][PubMed]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
StackebrandtE.,
GoebelB. M.1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. et al.1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]
YiH.,
SchumannP.,
ChunJ.2007; Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57:151–156 [View Article][PubMed]
YoonJ.-H.,
KimH.,
KimS.-B.,
KimH.-J.,
KimW. Y.,
LeeS. T.,
GoodfelloM.,
ParkY.-H.1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
YoonJ.-H.,
LeeS. T.,
ParkY.-H.1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
YoonJ.-H.,
KimI.-G.,
ShinD.-Y.,
KangK. H.,
ParkY.-H.2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]