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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 66, Issue 10

sp. nov., isolated from the soil of a tea plantation Free

Abstract

A novel bacterium, designated strain CF1, was isolated from a soil sample of a tea plantation and its taxonomic position was determined using a polyphasic approach. Strain CF1 was a Gram-stain-negative, facultatively anaerobic, non-sporulating, non-motile and rod-shaped bacterium. Optimum growth occurred at 25 °C and pH 6.0. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus , showing highest levels of similarity with respect to LMG 24238 (98.44 %). Additionally, strain CF1, LMG 24238 and LMG 27731 formed a distinct group in the phylogenetic tree based on 16S rRNA gene sequences. The predominant ubiquinone was Q-8, and the polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, two unidentified aminolipids and two unidentified polar lipids. The DNA G+C content was 60.2 mol%, and the major fatty acids were C, summed feature 8 (C 7 and/or C 6) and summed feature 3 (C 7 and/or C 6). The DNA–DNA relatedness values between strain CF1 and its close relatives including LMG 24238 and LMG 27731 49.3±0.4 % and 38.3±0.5 %, respectively. On the basis of phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CF1 (=LMG 28690=CGMCC 1.15103).

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Keyword(s): Paraburkholderia caffeinilytica sp. nov. and polyphasic taxonomy
© 2016 IUMS
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2016-10-01
2025-04-27
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Paraburkholderia caffeinilytica sp. nov., isolated from the soil of a tea plantation
Int J Syst Evol Microbiol 66, 4185 (2016); https://doi.org/10.1099/ijsem.0.001333
/content/journal/ijsem/10.1099/ijsem.0.001333
/content/journal/ijsem/10.1099/ijsem.0.001333
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