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Abstract

A bacterial strain, designated T16R-265, was isolated from rhizosphere of tomato in Buyeo-gun, Chungcheongnam-do, South Korea. The strain was Gram-stain-negative, aerobic, non-flagellated and rod- or long-rod-shaped. It grew at the range of 10–40 °C (optimum 28–30 °C), pH 6.0–9.0 (optimum pH 7.0) and with 0–3 % (w/v) NaCl (optimum 0 %). 16S rRNA gene sequence analysis showed that strain T16R-265 had the highest sequence similarity (98.0 %) with KIS20-3 and low sequence similarities of below 95 % with all other taxa. The phylogenetic tree revealed that the strain formed a robust monophyletic line with KIS20-3 separable from other clades. The predominant quinone of strain T16R-265 was MK-7, the polar lipids consisted of large amounts of phosphatidylethanolamine and an unknown lipid, and the major fatty acids were iso-C, iso-C 3-OH and iso-C G. DNA–DNA hybridization values with KACC 17319 were below 70 %. The DNA G+C content of strain T16R-265 is 46.2 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain T16R-265 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is T16R-265 (=KACC 18655=NBRC 111880). We also propose the reclassification of as comb. nov. (type strain KIS20-3=KACC 17319=JCM 19937).

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2016-10-01
2020-04-04
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