1887

Abstract

Root nodule bacteria were isolated from nodules on L. growing in neutral–alkaline soils from the Distrito Federal in central Brazil. The 16S rRNA gene sequence analysis of 10 strains placed them into the genus with the closest neighbouring species (each with 99 % similarity) being , , and . This high similarity, however, was not confirmed by multi-locus sequence analysis (MLSA) using three housekeeping genes (, and ), which revealed CCGE 501 to be the closest type strain (92 % sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with majority being C cyclo ω8 and summed feature 8 (Cω7/Cω6)], DNA G+C content (57.6 mol%), and carbon compound utilization patterns supported the placement of the novel strains in the genus . Results of average nucleotide identity (ANI) differentiated the novel strains from the closest species of the genus , , and with 89.0, 88.1 and 87.8 % similarity, respectively. The symbiotic genes essential for nodulation () and nitrogen fixation () were most similar (99–100 %) to those of , another -nodulating species. Based on the current data, these 10 strains represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is BR 10423 (=HAMBI 3664).

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2016-10-01
2024-12-13
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