1887

Abstract

A bacterial strain, designated CL-22, was isolated from an encrusting pore coral, Montipora aequituberculata, collected off the coast of Southern Taiwan. Its taxonomic position was investigated using a polyphasic approach. Cells of strain CL-22 were Gram-stain-negative, aerobic, motile by means of a single polar flagellum, rod-shaped and formed yellow colonies. Optimal growth occurred at 30 °C, pH 6.5−7 and in 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, showed that strain CL-22 fell into the clade comprising the type strains of species of the genus Thalassotalea . Strain CL-22 exhibited 16S rRNA gene sequence similarity values of 94.7–97.1 % to the type strains of species of the genus Thalassotalea . The major fatty acids (>10 %) of strain CL-22 were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The predominant isoprenoid quinone was Q-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The genomic DNA G+C content of strain CL-22 was 41.2 mol%. The differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain CL-22 should be classified as a novel species of the genus Thalassotalea ; the name Thalassotalea montiporae sp. nov. is proposed. The type strain is CL-22 (=LMG 24827=BCRC 17940).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001313
2016-10-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4077.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001313&mimeType=html&fmt=ahah

References

  1. Anzai Y., Kudo Y., Oyaizu H..( 1997;). The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. . Int J Syst Bacteriol 47: 249–251. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bowman J. P..( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. . Int J Syst Evol Microbiol 50: 1861–1868. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bowman J. P., McMeekin T. A..( 2005;). Order X. Alteromonadales ord. nov. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn.,vol. 2, Part B , pp. 443–490. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M.. New York, NY:: Springer;.[CrossRef]
    [Google Scholar]
  4. Chen W. M., Laevens S., Lee T. M., Coenye T., De Vos P., Mergeay M., Vandamme P..( 2001;). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51: 1729–1735. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cole J. R., Wang Q., Cardenas E., Fish J., Chai B., Farris R. J., Kulam-Syed-Mohideen A. S., McGarrell D. M., Marsh T. et al.( 2009;). The ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37: D141–D145. [CrossRef] [PubMed]
    [Google Scholar]
  6. Collins M. D..( 1994;). Isoprenoid quinones. . In Chemical Methods in Prokaryotic Systematics, pp. 265–309. Edited by Goodfellow M., O’Donnell A. G.. Chichester:: Wiley;.
    [Google Scholar]
  7. Embley T. M., Wait R..( 1994;). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics , pp. 121–161. Edited by Goodfellow M., O’Donnell A. G.. Chichester:: Wiley;.
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E..( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39: 224–229. [CrossRef]
    [Google Scholar]
  9. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein J..( 1993;). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences. . University of Washington, Seattle, USA:.
  11. Hall T. A..( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41: 95–98.
    [Google Scholar]
  12. Hosoya S., Adachi K., Kasai H..( 2009;). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59: 686–690. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hou T. T., Liu Y., Zhong Z. P., Liu H. C., Liu Z. P..( 2015;). Thalassotalea marina sp. nov., isolated from a marine recirculating aquaculture system, reclassification of Thalassomonas eurytherma as Thalassotalea eurytherma comb. nov. and emended description of the genus Thalassotalea. . Int J Syst Evol Microbiol 65: 4710–4715. [CrossRef] [PubMed]
    [Google Scholar]
  14. Jean W. D., Shieh W. Y., Liu T. Y..( 2006;). Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas. . Int J Syst Evol Microbiol 56: 1245–1250. [CrossRef] [PubMed]
    [Google Scholar]
  15. Jung Y.-T., Park S., Yoon J.-H..( 2014;). Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. . Antonie Van Leeuwenhoek 105: 81–87. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kimura M..( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;.[CrossRef]
    [Google Scholar]
  18. Kluge A. G., Farris J. S..( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool 18: 1–32. [CrossRef]
    [Google Scholar]
  19. Kumar S., Stecher G., Tamura K..( 2016;). mega7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. . Mol Biol Evol 33: 1870–1874. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B..( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39: 159–167. [CrossRef]
    [Google Scholar]
  21. Nokhal T. H., Schlegel H. G..( 1983;). Taxonomic study of Paracoccus denitrificans. . Int J Syst Bacteriol 33: 26–37. [CrossRef]
    [Google Scholar]
  22. Park S., Choi W. C., Oh T. K., Yoon J. H..( 2011;). Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. . Int J Syst Evol Microbiol 61: 2573–2576. [CrossRef] [PubMed]
    [Google Scholar]
  23. Park S., Jung Y.-T., Kang C.-H., Park J.-M., Yoon J.-H..( 2014;). Thalassotalea ponticola sp. nov., isolated from seawater, reclassification of Thalassomonas fusca as Thalassotalea fusca comb. nov. and emended description of the genus Thalassotalea. . Int J Syst Evol Microbiol 64: 3676–3682. [CrossRef] [PubMed]
    [Google Scholar]
  24. Powers E. M..( 1995;). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61: 3756–3758.[PubMed]
    [Google Scholar]
  25. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  26. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Newark, DE:: MIDI Inc;. http://www.microbialid.com/PDF/TechNote_101.pdf.
    [Google Scholar]
  27. Sun C., Huo Y.-Y., Liu J.-J., Pan J., Qi Y.-Z., Zhang X.-Q., Zhang Y., Zheng G., Wu M..( 2014;). Thalassomonas eurytherma sp. nov., a marine proteobacterium. . Int J Syst Evol Microbiol 64: 2079–2083. [CrossRef] [PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G..( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25: 4876–4882. [CrossRef]
    [Google Scholar]
  29. Thompson F. L., Barash Y., Sawabe T., Sharon G., Swings J., Rosenberg E..( 2006;). Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. . Int J Syst Evol Microbiol 56: 365–368. [CrossRef] [PubMed]
    [Google Scholar]
  30. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R..( 2007;). Phenotypic characterization and the principles of comparative systematic. . In Methods for General and Molecular Bacteriology, , 3rd edn. , pp. 330–393 . Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R.. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H, Moore W. E. C., Murray R. G. E. et al.( 1987;). Report of the hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Evol Microbiol 37: 463–464. [CrossRef]
    [Google Scholar]
  32. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J..( 1991;). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173: 697–703.[PubMed]
    [Google Scholar]
  33. Wen C. M., Tseng C. S., Cheng C. Y., Li Y. K..( 2002;). Purification, characterization and cloning of a chitinase from Bacillus sp. NCTU2. . Biotechnol Appl Biochem 35: 213–219. [CrossRef] [PubMed]
    [Google Scholar]
  34. Yi H., Bae K. S., Chun J..( 2004;). Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54: 377–380. [CrossRef] [PubMed]
    [Google Scholar]
  35. Zhang Y., Tang K., Shi X., Zhang X.-H..( 2014;). Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas. . Int J Syst Evol Microbiol 64: 1223–1228. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001313
Loading
/content/journal/ijsem/10.1099/ijsem.0.001313
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error