1887

Abstract

A bacterial strain, RS-LYSO-3, was isolated from tobacco-cultivated soil, collected near Chuxiong, Yunnan province, southwestern China. RS-LYSO-3 could effectively inhibit the invasion of powdery mildew on tobacco. The colonies of RS-LYSO-3 were pale yellow, and its cells were Gram-stain-negative and rod-shaped, with 68 mol% DNA G+C content. Gene sequence analysis for its 16S rRNA gene revealed the highest similarity (97.78 %) with that of KMM 329. Chemotaxonomic data showed that RS-LYSO-3 possesses a quinone system with Q-8, and iso-C, summed feature 9 and iso-C as the predominant fatty acids, all of which support the affiliation of RS-LYSO-3 to the genus . The results of DNA–DNA hybridization, physiological and biochemical tests clearly proved that RS-LYSO-3 is a representative of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RS-LYSO-3 (=CCIC 23922=JCM 31042).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001303
2016-10-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/4016.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001303&mimeType=html&fmt=ahah

References

  1. Bae H.-S., Im W.-T., Lee S.-T. 2005; Lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. Int J Syst Evol Microbiol 55:1155–1161 [View Article][PubMed]
    [Google Scholar]
  2. Christensen P., Cook F. D. 1978; Lysobacter, a new genus of nonhiting, gliding bacteria with a high base Ratio. Int J Syst Evol Microbiol 28:367–393
    [Google Scholar]
  3. Geng G. 2011; Effect of crop root exudates on carbon and nitrogen contents, microorganisms quatity and enzyme activities in soil. In Soil and Environment College. (Shengyang: Shengyang Agricultural University);
  4. Gordon R. E., Smith M. M. 1955; Proposed group of characters for the separation of Streptomyces and nocardia. J Bacteriol 69:147–150[PubMed]
    [Google Scholar]
  5. Gordon R. E. 1966; Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45:355–364 [View Article][PubMed]
    [Google Scholar]
  6. Gordon R. E. 1967; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria pp. 293–321 Edited by Gray T. R. G., Parkinson B. Liverpool: Liverpool University Press;
    [Google Scholar]
  7. Hayward A. C., Fegan N., Fegan M., Stirling G. R. 2010; Stenotrophomonas and Lysobacter: ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology. J Appl Microbiol 108:756–770 [View Article][PubMed]
    [Google Scholar]
  8. Ji G.-H. 2011; Advancesin the study on Lysobacter spp. bacteria andtheir effects on biological control of plant diseases. J Yunnan Agric Univ 26:124–130
    [Google Scholar]
  9. Jiang W.-T. 2012 Taxonomyand flora of powdery mildews (Erysiphaceae) in Jilin Province. In Agricultural College (Jilin: Jilin Agricultural University);
    [Google Scholar]
  10. Jung H.-M., Leonid N.-T., Im W.-T., Yoo S.-A., Lee S.-T. 2008; Lysobacter ginsengisoli sp. nov., a novel species isolated from soil in Pocheon Province, South Korea. J Microbiol Biotechnol 18:1496–1499[PubMed]
    [Google Scholar]
  11. Lane D. J. 1991; 16S/23SrRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  12. Lin W.-M. 1990 Moleculargenetic Classification and Identification of Bacteria Shanghai: Shanghai Science and Technology Press;
    [Google Scholar]
  13. Lee J.-W., Im W.-T., Myung K.-K., Yang D.-C. 2006; Lysobacter koreensis sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 56:231–235 [View Article][PubMed]
    [Google Scholar]
  14. Li Y.-M., Zhang Z.-K., Jia Y.-T., Shen Y.-M., He H.-P., Fang R.-X., Chen X.-Y., Hao X.-J., Hao X.-J. 2008; 3-Acetonyl-3-hydroxyoxindole: a new inducer of systemic acquired resistance in plants. Plant Biotechnol J 6:301–308 [View Article][PubMed]
    [Google Scholar]
  15. Lin Y.-C., Uemori K., de Briel D. A., Arunpairojana V., Yokota A. 2004; Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 54:1669–1676 [View Article][PubMed]
    [Google Scholar]
  16. Luo G.-S., Shi Z.-J., Wang G.-J. 2012; Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. Int J Syst Evol Microbiol 62:1659–1665 [View Article][PubMed]
    [Google Scholar]
  17. Lyudmila A. R., Masataka U., Naoto T., Galina M. F., Valery V. M. 2008; Lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. Int J Syst Evol Microbiol 58:370–374 [View Article][PubMed]
    [Google Scholar]
  18. Muhammad Z. S., Im W.-T. 2016; Lysobacter hankyongensis sp. nov., isolated from activated sludge and Lysobacter Sediminicola sp. nov., isolated from fresh water sediment. Int J Syst Evol Microbiol 66:212–218 [View Article][PubMed]
    [Google Scholar]
  19. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid byhigh-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  20. Park J. H., Kim R., Aslam Z., Jeon C. O., Chung Y. R. 2008; Lysobacter capsici sp. nov., with antimicrobial activity, isolated from the rhizosphere of pepper, and emended description of the genus Lysobacter. Int J Syst Evol Microbiol 58:387–392 [View Article][PubMed]
    [Google Scholar]
  21. Reichenbach H. 2006; The genus Lysobacter. Prokaryotes 6:939–957
    [Google Scholar]
  22. Ren G.-W., Kong F.-Y., Wang F.-L. 2009 GB/T23222-2008 Grade and invetigation method of tobacco diseases and insect pests Beijing: Standards Press of China;
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  24. Srinivasan S., Kim M. K., Sathiyaraj G., Kim H.-B., Kim Y.-J., Yang D.-C. 2010; Lysobacter soli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 60:1543–1547 [View Article][PubMed]
    [Google Scholar]
  25. Tindall B. J. 1990; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202 [View Article]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  27. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  28. Yang S.-Z., Feng G.-D., Zhu H.-H., Wang Y.-H. 2015; Lysobacter mobilis sp. nov., isolated from abandoned lead-zinc ore. Int J Syst Evol Microbiol 65:833–837 [View Article][PubMed]
    [Google Scholar]
  29. Yassin A. F., Chen W.-M., Hupfer H., Siering C., Kroppenstedt R. M., Arun A. B., Lai W.-A., Shen F.-T., Rekha P. D., Young C. C. 2007; Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57:1131–1136 [View Article][PubMed]
    [Google Scholar]
  30. Ye X.-M., Chu C.-W., Shi C., Zhu J.-C., He Q., He J. 2015; Lysobacter caeni sp. nov., isolated from the sludge of a pesticide manufacturing factory. Int J Syst Evol Microbiol 65:845–850 [View Article][PubMed]
    [Google Scholar]
  31. Zhang L., Bai J., Wang Y., Wu G.-L., Dai J., Fang C.-X. 2011; Lysobacter korlensis sp. nov. and Lysobacter bugurensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 61:2259–2265 [View Article][PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001303
Loading
/content/journal/ijsem/10.1099/ijsem.0.001303
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error