1887

Abstract

Eight strains representing three novel yeast species were isolated from insects distributed in three localities in Nanyang, Henan Province, Central China during 2014 and 2015. Sequence analysis of the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that these species are members of the clade. These three novel species have a greater than 2.5 % difference from each other or their closest known species in the D1/D2 sequences. The three yeast species can also be separated from their closest known species in terms of physiological characteristics. Moreover, a sexual state could not be found in these three novel yeast species on various sporulation media. Therefore, the three novel species are described as f.a., sp. nov. (type strain, NYNU 15885=CICC 33092=CBS 14176), f.a., sp. nov. (type strain, NYNU 15896=CICC 33093=CBS 14177) and f.a., sp. nov. (type strain, NYNU 146103=CICC 33062=CBS 13929). The MycoBank numbers of f.a., sp. nov., f.a., sp. nov. and f.a., sp. nov. are MB 816962, MB 816963 and MB 816964, respectively.

Keyword(s): insect , Wickerhamiella and yeast
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001300
2016-10-01
2024-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3995.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001300&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blas t and PSI-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [View Article][PubMed]
    [Google Scholar]
  2. Badotti F., Silva P. A. B., Mendonça M. C., Gomes F. C., Morais P. B., Lachance M. A., Rosa C. A. 2013; Wickerhamiella dulcicola sp. nov. and Wickerhamiella cachassae sp. nov., yeasts isolated from cachaça fermentation in Brazil. Int J Syst Evol Microbiol 63:1169–1173 [View Article][PubMed]
    [Google Scholar]
  3. Barbosa A. C., Morais C. G., Morais P. B., Rosa L. H., Pimenta R. S., Lachance M. A., Rosa C. A. 2012; Wickerhamiella pagnoccae sp. nov. and Candida tocantinsensis sp. nov., two ascomycetous yeasts from flower bracts of Heliconia psittacorum (Heliconiaceae). Int J Syst Evol Microbiol 62:459–464 [View Article][PubMed]
    [Google Scholar]
  4. Dayo-Owoyemi I., Rosa C. A., Rodrigues A., Pagnocca F. C. 2014; Wickerhamiella kiyanii f.a., sp. nov. and Wickerhamiella fructicola f.a., sp. nov., two yeasts isolated from native plants of Atlantic rainforest in Brazil. Int J Syst Evol Microbiol 64:2152–2158 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  6. Hagler A. N., Ribeiro J. R., Pinotti T., Brandão L. R., Pimenta R. S., Lins U., Lee C. F., Hsieh C. W., Lachance M. A., Rosa C. A. 2013; Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates. Int J Syst Evol Microbiol 63:3099–3103 [View Article][PubMed]
    [Google Scholar]
  7. Ji Z. H., Jia J. H., Bai F. Y. 2009; Four novel Candida species in the Candida albicans/Lodderomyces elongisporus clade isolated from the gut of flower beetles. Antonie Van Leeuwenhoek 95:23–32 [View Article][PubMed]
    [Google Scholar]
  8. Khunnamwong P., Surussawadee J., Jindamorakot S., Limtong S. 2014; Wickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf. Int J Syst Evol Microbiol 64:3849–3855 [View Article][PubMed]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  10. Kurtzman C. P., Robnett C. J. 1998; Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 73:331–371[PubMed] [CrossRef]
    [Google Scholar]
  11. Kurtzman C. P., Robnett C. J. 2007; Multigene phylogenetic analysis of the Trichomonascus, Wickerhamiella and Zygoascus yeast clades, and the proposal of Sugiyamaella gen. nov. and 14 new species combinations. FEMS Yeast Res 7:141–151 [View Article][PubMed]
    [Google Scholar]
  12. Kurtzman C. P., Robnett C. J. 2013; Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res 13:23–33 [View Article][PubMed]
    [Google Scholar]
  13. Kurtzman C. P., Fell J. W., Boekhout T., Robert V. 2011; Methods for isolation, phenotypic characterization and maintenance of yeasts. In The Yeasts, a Taxonomic Study, 5th edn. vol. 1 , pp. 87–110 Edited by Kurtzman C. P., Fell J. W., Bekhout T. San Diego, CA: Elsevier; [CrossRef]
    [Google Scholar]
  14. Lachance M. A., Kurtzman C. P. 2011; Wickerhamiella van der Walt (1973). In The Yeasts: A Taxonomic Study, 5th edn. vol. 2 , pp. 891–897 Edited by Kurtzman C. P., Fell J. W., Boekhout T. San Diego, CA: Elsevier; [CrossRef]
    [Google Scholar]
  15. Lachance M. A., Boekhout T., Scorzetti G., Fell J. W., Kurtzman C. P. 2011; Candida Berkhout (1923). In The Yeasts, a Taxonomic Study, 5th edn. vol. 2 , pp. 987–1278 . Edited by Kurtzman C. P., Fell J. W., Boekhout T. San Diego, CA: Elsevier; [CrossRef]
    [Google Scholar]
  16. Nakase T., Jindamorakot S., Limtong S., Am-in S., Kawasaki H., Imanishi Y., Potacharoen W., Tanticharoen M. 2007; Candida kazuoi sp. nov. and Candida hasegawae sp. nov., two new species of ascomycetous anamorphic yeasts isolated from insect frass in Thailand. J Gen Appl Microbiol 53:239–245 [View Article][PubMed]
    [Google Scholar]
  17. Nguyen N. H., Suh S. O., Marshall C. J., Blackwell M. 2006; Morphological and ecological similarities: wood-boring beetles associated with novel xylose-fermenting yeasts, Spathaspora passalidarum gen. sp. nov. and Candida jeffriesii sp. nov. Mycol Res 110:1232–1241 [View Article][PubMed]
    [Google Scholar]
  18. Ren Y. C., Wang Y., Chen L., Ke T., Hui F. L. 2014; Wickerhamiella allomyrinae f.a., sp. nov., a yeast species isolated from the gut of the rhinoceros beetle Allomyrina dichotoma . Int J Syst Evol Microbiol 64:3856–3861 [View Article][PubMed]
    [Google Scholar]
  19. Ren Y. C., Xu L. L., Zhang L., Hui F. L. 2015; Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles. Int J Syst Evol Microbiol 65:3580–33585 [View Article][PubMed]
    [Google Scholar]
  20. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011; mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  22. Trindade R. C., Resende M. A., Silva C. M., Rosa C. A. 2002; Yeasts associated with fresh and frozen pulps of Brazilian tropical fruits. Syst Appl Microbiol 25:294–300 [View Article][PubMed]
    [Google Scholar]
  23. Urbina H., Schuster J., Blackwell M. 2013; The gut of Guatemalan passalid beetles: a habitat colonized by cellobiose- and xylose-fermenting yeasts. Fungal Ecology 6:339–355 [View Article]
    [Google Scholar]
  24. White T. J., Bruns T., Lee S., Taylor J. W. 1990; Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications , pp. 315–322 . Edited by Innis M. A., Gelfan D. H., Sninsky J. J., White T. J. New York, NY: Academic Press;
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.001300
Loading
/content/journal/ijsem/10.1099/ijsem.0.001300
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error