1887

Abstract

A novel endophytic actinobacterium, designated strain CPCC 204076, was isolated from surface-sterilized tissue of the medicinal plant (Thunb.) collected from Sichuan Province, south-west China. The taxonomic position of the isolate was investigated by a polyphasic approach. The strainwas aerobic, Gram-stain-positive, non-motile, non-spore-forming and rod-shaped. Growth was observed at 10–37 °C, at pH 5.0–10.0 and with 0–3.0 % (w/v) NaCl. The polar lipid fraction consisted of diphosphatidylglycerol, a phospholipid, an aminolipid, a glycolipid, an aminophospholipid and phosphatidylinositol. The cell wall contained -diaminopimelic acid as the diagnostic diamino acid and the peptidoglycan was of type A4γ. The menaquinone system consisted of MK-9(H) and MK-8(H). The major cellular fatty acids (>10 %) were iso-C and anteiso-C. The genomic DNA G+C content of strain CPCC 204076 was found to be 71.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that CPCC 204076 belongs to the genus with highest sequence similarity to DSM 45627 (96.5 %), DSM 45908 (96.5 %) and JCM 30448 (96.1 %), and much lower similarities (<95.0 %) to other available 16S rRNA gene sequences from validly described pure cultures. However, DNA–DNA hybridyzation values between strain CPCC 204076 and the three recognized species were 31±3.1 % ( DSM 45627), 33±2.9 % ( DSM 45908) and 37±1.7 % ( JCM 30448), which were all far below the recommended cut-off value of 70 %. The phenotypic and genomic characteristics distinctly indicated that strain CPCC 204076 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CPCC 204076 (I13A-01604) (=DSM 46866=NBRC 110718).

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2016-10-01
2021-10-15
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