1887

Abstract

A novel Gram-stain-negative, small rod-shaped, motile strain, designated M1, was isolated from hydrocarbon-contaminated desert sands collected from Kuwait. M1 grew at 12–45 °C, pH 6.1–8.8 and 0–4.5 % (w/v) NaCl concentration. Casein, Tween-20 and Tween-80 were hydrolyzed while starch, urea, chitin, DNA and carboxymethyl-cellulose were not hydrolyzed by M1. C18 : 1ω6c/C18 : 1ω7c, C16 : 0 and C12 : 0 aldehyde were predominant fatty acids with minor amounts of iso-C16 : 1I/C14 : 0 3-OH, C16 : 0 3-OH, C18 : 0, C16 : 1ω6c/C16 : 1ω7c, C16 : 1ω11c and C18 : 1ω9c. Diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and phosphatidylcholine (PC) were the major polar lipids. Minor amounts of unidentified aminolipids (AL1–2), unidentified phospholipids (PL1–2) and unidentified lipids (L1–4) were also detected. Genomic G+C content was 69.5 mol%. Comparisons of 16S rRNA gene sequence indicate that M1 represent a member of the genus Skermanella in the family Rhodospirillaceae of the class Alphaproteobacteria . M1 has a sequence similarity of 97.5 % with Skermanella aerolata 5416T-32, 96.7 % with Skermanella stibiiresistens SB22 and <95.7 % with the other two species of the genus Skermanella . DNA–DNA hybridization values between M1 and the type strains of the most closely related species were clearly below the 70 % threshold. Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain M1 as a representative of a novel species in the genus Skermanella , for which the name Skermanella rosea sp. nov. is proposed. The type strain is M1 (=KEMB 2255-458=JCM 31276).

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2016-10-01
2019-10-16
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