sp. nov., isolated from blood culture and peritoneal dialysis effluent of patients in Hong Kong Free

Abstract

Two bacterial strains, HKU50 and HKU46, were isolated in Hong Kong from the blood culture and the peritoneal dialysis effluent of two patients. The strains are Gram-stain-positive, acid-fast, non-motile, non-sporulating bacilli. They grow on Columbia agar with 5 % defibrinated sheep blood and brain–heart infusion agar under aerobic conditions with 5 % CO at 37 °C as pink-to-orange, non-haemolytic colonies. The strains are catalase-positive and oxidase-negative, and have a unique biochemical profile distinguishable from other closely related species. DNA sequencing revealed that both isolates possessed multiple intra-genomic 16S rRNA gene copies (99.8–100 % sequence identities to NRRL B-24551 and NRRL B-16283). Phylogenetic analysis of the 16S rRNA gene, and showed that the two isolates formed a distinct branch within the genus and were most closely related to and . DNA–DNA hybridization demonstrated ≤53.7 % and ≤49.4 % DNA relatedness between the two isolates and , and between the two isolates and , respectively. Hierarchical cluster analysis of MALDI–TOF MS main spectrum profiles showed that strains HKU50 and HKU46 were closely related to each other, but were distinct from , , or any other species of the genus in the Bruker database. The chemotaxonomic traits of the two strains were highly similar, and the major fatty acids were summed feature 4 (iso-C 2-OH/C -9), C, C -9, and tuberculostearic acid. A novel species named sp. nov. is proposed to accommodate strains HKU50 and HKU46, with strain HKU50 (=CCOS 955=CIP 111027=NBRC 111234=NCCP 16210) as the type strain.

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2016-10-01
2024-03-29
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