1887

Abstract

Two bacterial strains, HKU50 and HKU46, were isolated in Hong Kong from the blood culture and the peritoneal dialysis effluent of two patients. The strains are Gram-stain-positive, acid-fast, non-motile, non-sporulating bacilli. They grow on Columbia agar with 5 % defibrinated sheep blood and brain–heart infusion agar under aerobic conditions with 5 % CO at 37 °C as pink-to-orange, non-haemolytic colonies. The strains are catalase-positive and oxidase-negative, and have a unique biochemical profile distinguishable from other closely related species. DNA sequencing revealed that both isolates possessed multiple intra-genomic 16S rRNA gene copies (99.8–100 % sequence identities to NRRL B-24551 and NRRL B-16283). Phylogenetic analysis of the 16S rRNA gene, and showed that the two isolates formed a distinct branch within the genus and were most closely related to and . DNA–DNA hybridization demonstrated ≤53.7 % and ≤49.4 % DNA relatedness between the two isolates and , and between the two isolates and , respectively. Hierarchical cluster analysis of MALDI–TOF MS main spectrum profiles showed that strains HKU50 and HKU46 were closely related to each other, but were distinct from , , or any other species of the genus in the Bruker database. The chemotaxonomic traits of the two strains were highly similar, and the major fatty acids were summed feature 4 (iso-C 2-OH/C -9), C, C -9, and tuberculostearic acid. A novel species named sp. nov. is proposed to accommodate strains HKU50 and HKU46, with strain HKU50 (=CCOS 955=CIP 111027=NBRC 111234=NCCP 16210) as the type strain.

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2016-10-01
2020-01-22
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