1887

Abstract

A Gram-stain-negative, aerobic, non-flagellated and pleomorphic bacterium, designated HJR-2, was isolated from a junction between the ocean and a freshwater lake on the East Sea, South Korea. It grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 0–1.5 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain HJR-2 clustered with the type strains of and and this cluster fell within the clade comprising the type strains of species. Strain HJR-2 exhibited 16S rRNA gene sequence similarity values of 97.2 and 97.0 % to the type strains of and , respectively, and 93.9–96.2 % to the type strains of species. Strain HJR-2 contained Q-10 as the predominant ubiquinone and C 7, 11-methyl C 7 and C as major fatty acids. Major polar lipids of strain HJR-2 were phosphatidylglycerol and two unidentified glycolipids. The DNA G+C content was 66.0 mol% and its mean DNA–DNA relatedness values with the type strains of and were 11–17 %. Differential phenotypic properties, together with its phylo genetic and genetic distinctiveness, revealed that strain HJR-2 is separated from , and species. On the basis of the data presented, strain HJR-2 (=KCTC 52211=NBRC 112271) is considered to be the type strain of a novel species of the genus , for which the name sp. nov. is proposed. In this study, it is also proposed that and be reclassified as members of the genus .

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2016-10-01
2020-09-25
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