1887

Abstract

A novel facultatively anaerobic, rod-shaped bacterium, designated LAM1188, was isolated from the roots of rice () in Hubei Province. Cells of LAM1188 were Gram-stain-negative and motile. The temperature and pH ranges for growth were 15–40 °C (optimum: 30 °C) and pH 5–10 (optimum: pH 7), respectively. The strain did not require NaCl for growth but tolerated up to 3.5 % NaCl (w/v). Analysis of the 16S rRNA gene sequence indicated that the isolate represented a member of the genus , and was most closely related to MDA0585 and CC-SEYA-1 with 98.7 % and 97.3 % sequence similarity, respectively. The values of DNA–DNA hybridization between LAM1188 and JCM 14163 and CCUG 55175 were 54.0±2.1 % and 44.0±1.2 %, respectively. The major cellular fatty acids were C and summed feature 3 (Cω6 and/or Cω7). The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, four unidentified aminolipids and four unidentified lipids. The respiratory quinone was ubiquinone Q-8. The DNA G+C content was 64.1 mol% as determined by the method. On the basis of its phenotypic, chemotaxonomic and phylogenetic characteristics, strain LAM1188 is suggested to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LAM1188 (=ACCC 19900=JCM 31180).

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2016-10-01
2021-07-25
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