1887

Abstract

A Gram-stain-positive, catalase-negative, coccus-shaped organism was isolated from oral cavity samples collected from healthy elephants. The isolated strain, NUM 2404, was tentatively identified as a streptococcal species based on the results of biochemical tests. Although a comparative 16S rRNA gene sequence analysis suggested the classification of this organism into the genus Streptococcus , it did not correspond to any recognized species of the genus. Strain NUM 2404 was related most closely to Streptococcus saliviloxodontae NUM 6306 with 95.8 % 16S rRNA gene sequence similarity, but the phylogenetic tree reconstructed based on the 16S rRNA gene sequence showed that NUM 2404 clustered with Streptococcus mutans NCTC 10449 and Streptococcus troglodytae TKU 31. Comparative sequence analysis based on two housekeeping genes, groEL, which encodes the 60 kDa heat-shock protein, and rpoB, encoding the β subunit of RNA polymerase, of NUM 2404 indicated that it was most closely related to those of Streptococcus orisratti A63 and Streptococcus sobrinus ATCC 33478 with 82.7 and 85.1 % sequence similarities, respectively. On the basis of genotypic and phenotypic differences, it is proposed that the novel isolate be classified in the genus Streptococcus as representative of a novel species, Streptococcus dentiloxodontae sp. nov. The type strain is NUM 2404 (=JCM 19284=DSM 27381).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001280
2016-10-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3878.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001280&mimeType=html&fmt=ahah

References

  1. Glazunova O. O., Raoult D., Roux V..( 2009;). Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus. . Int J Syst Evol Microbiol 59: 2317–2322. [CrossRef] [PubMed]
    [Google Scholar]
  2. Hiraishi A..( 1992;). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. . Lett Appl Microbiol 15: 210–213. [CrossRef] [PubMed]
    [Google Scholar]
  3. Hirasawa M., Takada K..( 1994;). Porphyromonas gingivicanis sp. nov. and Porphyromonas crevioricanis sp. nov., isolated from beagles. . Int J Syst Bacteriol 44: 637–640. [CrossRef] [PubMed]
    [Google Scholar]
  4. Hirasawa M., Kiyono H., Shiota T., Michalek S. M., McGhee J. R..( 1980;). Virulence of Streptococcus mutans: immunochemical characterization of a serotyp g-defective mutant (C307). . Infect Immun 27: 697–699.[PubMed]
    [Google Scholar]
  5. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  6. Liò P., Goldman N..( 1998;). Models of molecular evolution and phylogeny. . Genome Res 8: 1233–1244.[PubMed]
    [Google Scholar]
  7. Nakano K., Ooshima T..( 2009;). Serotype classification of Streptococcus mutans and its detection outside the oral cavity. . Future Microbiol 4: 891–902. [CrossRef] [PubMed]
    [Google Scholar]
  8. Okamoto M., Imai S., Miyanohara M., Saito W., Momoi Y., Abo T., Nomura Y., Ikawa T., Ogawa T. et al.( 2013;). Streptococcus troglodytae sp. nov., from the chimpanzee oral cavity. . Int J Syst Evol Microbiol 63: 418–422. [CrossRef] [PubMed]
    [Google Scholar]
  9. Ozeki Y..( 2011;). Characterization of a large amount of water-insoluble polysaccharide synthesis Streptococcus salivarius. . Int J Oral-Med Sciences 10: 25–30. [CrossRef]
    [Google Scholar]
  10. Saito M., Shinozaki-Kuwahara N., Hirasawa M., Takada K..( 2014;). Streptococcus loxodontisalivarius sp. nov. and Streptococcus saliviloxodontae sp. nov., isolated from oral cavities of elephants. . Int J Syst Evol Microbiol 64: 3288–3292. [CrossRef] [PubMed]
    [Google Scholar]
  11. Saito M., Shinozaki-Kuwahara N., Hirasawa M., Takada K..( 2015;). Streptococcus oricebi sp. nov., isolated from the oral cavity of tufted capuchin. . Int J Syst Evol Microbiol 66: 1063–1067. [CrossRef] [PubMed]
    [Google Scholar]
  12. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  13. Shinozaki-Kuwahara N., Takada K., Hirasawa M..( 2011;). Streptococcus ursoris sp. nov., isolated from the oral cavities of bears. . Int J Syst Evol Microbiol 61: 40–44. [CrossRef] [PubMed]
    [Google Scholar]
  14. Shinozaki-Kuwahara N., Saito M., Hirasawa M., Takada K..( 2014;). Streptococcus oriloxodontae sp. nov., isolated from the oral cavities of elephants. . Int J Syst Evol Microbiol 64: 3755–3759. [CrossRef] [PubMed]
    [Google Scholar]
  15. Shiroza T., Shinozaki N., Watanabe T., Ikemi T., Fukushima K., Abiko Y..( 1998;). Rapid isolation of chromosomal DNA from oral streptococci and polymerase chain reaction-oriented restriction fragment-length polymorphism analysis for genetic heterogeneity. . Oral Microbiol Immunol 13: 11–16. [CrossRef] [PubMed]
    [Google Scholar]
  16. Stackebrandt E., Ebers J..( 2006;). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33: 152–155.
    [Google Scholar]
  17. Tabchoury C. P., Sousa M. C., Arthur R. A., Mattos-Graner R. O., Del Bel Cury A. A., Cury J. A..( 2008;). Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers. . J Appl Oral Sci 16: 403–407. [CrossRef] [PubMed]
    [Google Scholar]
  18. Takada K., Hirasawa M..( 2007;). Streptococcus orisuis sp. nov., isolated from the pig oral cavity. . Int J Syst Evol Microbiol 57: 1272–1275. [CrossRef] [PubMed]
    [Google Scholar]
  19. Takada K., Hirasawa M..( 2008;). Streptococcus dentirousetti sp. nov., isolated from the oral cavities of bats. . Int J Syst Evol Microbiol 58: 160–163. [CrossRef] [PubMed]
    [Google Scholar]
  20. Takada K., Wyszomirska J., Shiota T..( 1984;). Serological characterization of Streptococcus mutans serotype polysaccharide g and its different molecular weight forms. . Infect Immun 45: 464–469.[PubMed]
    [Google Scholar]
  21. Takada K., Hirasawa M..( 2010;). Streptococcus dentapri sp. nov., isolated from the wild boar oral cavity. . Int J Syst Evol Microbiol 60: 820–823. [CrossRef] [PubMed]
    [Google Scholar]
  22. Takada K., Saito M., Tsudukibashi O., Hiroi T., Hirasawa M..( 2013;). Streptococcus orisasini sp. nov. and Streptococcus dentasini sp. nov., isolated from the oral cavity of donkeys. . Int J Syst Evol Microbiol 63: 2782–2786. [CrossRef] [PubMed]
    [Google Scholar]
  23. Tamura K., Nei M..( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol 10: 512–526.
    [Google Scholar]
  24. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S..( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Whiley R. A., Beighton D..( 1998;). Current classification of the oral streptococci. . Oral Microbiol Immunol 13: 195–216. [CrossRef] [PubMed]
    [Google Scholar]
  26. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R..( 2008;). The all-species living tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. . Syst Appl Microbiol 31: 241–250. [CrossRef] [PubMed]
    [Google Scholar]
  27. Zhu H., Willcox M. D., Knox K. W..( 2000;). A new species of oral Streptococcus isolated from Sprague-Dawley rats, Streptococcus orisratti sp. nov. . Int J Syst Evol Microbiol 50: 55–61. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001280
Loading
/content/journal/ijsem/10.1099/ijsem.0.001280
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error