1887

Abstract

A Gram-stain-negative, rod-shaped, endospore-forming bacterium, strain LOOC204, was isolated from corn silage that had been prepared and stored in Hokkaido, Japan. The isolate grew well at 4–37 °C and at pH 6.0–8.5. The major cellular fatty acids were anteiso-C, C, iso-C and iso-C. The major menaquinone was MK-7. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminophospholipids, one unidentified phospholipid, two unidentified aminolipids and three unidentified lipids were detected as the cellular polar lipids. The genomic DNA G+C content of LOOC204 was 52.2±0.7 mol%. A comparative 16S rRNA gene sequence analysis showed that LOOC204 represented a member of the genus , and high levels of sequence similarity of LOOC204 with KK19 (97.5 %) and Be17 (97.4 %) were found. Based on sequence analysis of the RNA polymerase β subunit () gene, LOOC204 was clearly separated from its phylogenetic neighbors in the genus . DNA–DNA hybridisation experiments showed that LOOC204 displayed less than 40 % reassociation values toward its nearest phylogenetic neighbours of the genus , indicating the unique taxonomic status of LOOC204. Based on these data, we conclude that strain LOOC204 represents a novel species of the genus , named sp. nov. The type strain is LOOC204 (=JCM 30974=DSM 101953).

Keyword(s): corn silage , New Taxa and Paenibacillus
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2016-10-01
2020-10-01
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