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Abstract

A novel Gram-stain-positive, aerobic, non-motile actinobacterium, designated strain WP1, was isolated from a deep-sea sediment sample collected from the Indian Ocean, and examined in a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WP1 formed a distinct phyletic lineage in the genus and was closely related to KT2025 (97.8 % 16S rRNA gene sequence similarity), 1BDZ (97.3 %) and Ms392A (97.2 %). The isolate grew at 4–45 °C, pH 5–11 and in the presence of 0–8 % (w/v) NaCl. The cell wall of the novel strain contained -diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids identified were iso-C, Cω8 and Cω6. The predominant menaquinones were MK-9(H) and MK-7. The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylmethylethanolamine, four unknown phospholipids and one unknown glycolipid. The genomic DNA G+C content was 72.2 mol%. Strain WP1 displayed low DNA–DNA reassociation with DSM 45510 (mean value 36.2 %), JCM 12460 (31.5 %) and JCM 16121 (29.7 %). Based on the data reported here, strain WP1 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is WP1 (=KCTC 39642=MCCC 1A10745).

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2016-10-01
2022-01-21
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