1887

Abstract

A novel single-spore-producing actinomycete, designated strain 2H-TWYE14, was isolated from the head of an ant ( Mayr) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 2H-TWYE14 belongs to the genus , with highest sequence similarity to NRRL 2466 (98.84 %). Analysis based on the gene also indicated that strain 2H-TWYE14 should be assigned to the genus . The chemotaxonomic properties of strain 2H-TWYE14 were consistent with those of members of the genus . The cell wall contained -diaminopimelic acid. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C and iso-C. DNA–DNA hybridization experiments and phenotypic tests were carried out between strain 2H-TWYE14 and its phylogenetically closely related strain JCM 4251, which further clarified their relatedness and demonstrated that 2H-TWYE14 could be distinguished from . Therefore, it is concluded that strain 2H-TWYE14 can be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2H-TWYE14 (=DSM 100523=CGMCC 4.7275).

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2016-10-01
2020-09-25
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References

  1. Collins M. D.. 1985; Isoprenoid quinone analyses in bacterial classification and identification. In Chemical Methods in Bacterial Systematics pp267–284 Edited by Goodfellow M., Minnikin D. E.. London: Academic Press;
    [Google Scholar]
  2. Coombs J. T., Franco C. M.. 2003; Isolation and identification of actinobacteria from surface-sterilized wheat roots. Appl Environ Microbiol69:5603–5608 [CrossRef][PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A.. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem12:133–142 [CrossRef][PubMed]
    [Google Scholar]
  4. Felsenstein J.. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  5. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–791 [CrossRef]
    [Google Scholar]
  6. Gao R., Liu C., Zhao J., Jia F., Yu C., Yang L., Wang X., Xiang W.. 2014; Micromonospora jinlongensis sp. nov., isolated from muddy soil in China and emended description of the genus Micromonospora. Antonie Van Leeuwenhoek105:307–315 [CrossRef][PubMed]
    [Google Scholar]
  7. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C.. 1974; Nocardia coeliaca, Nocardia autotrophica, and the Nocardin Strain. Int J Syst Bacteriol24:54–63 [CrossRef]
    [Google Scholar]
  8. Hatano K., Nishii T., Kasai H.. 2003; Taxonomic re-evaluation of whorl-forming Streptomyces (formerly Streptoverticillium) species by using phenotypes, DNA-DNA hybridization and sequences of gyrB, and proposal of Streptomyces luteireticuli (ex Katoh and Arai 1957) corrig., sp. nov., nom. rev. Int J Syst Evol Microbiol53:1519–1529 [CrossRef][PubMed]
    [Google Scholar]
  9. Huss V. A., Festl H., Schleifer K. H.. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol4:184–192 [CrossRef][PubMed]
    [Google Scholar]
  10. Jones K. L.. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol57:141–145
    [Google Scholar]
  11. Kämpfer P.. 2012; Genus I. Streptomyces. In Bergey’s Manual of Systematic Bacteriology, the Actinobacteria1455–1767 Edited by Goodfellow M., Kämpfer P., Busse H.-J., Trujillo M. E., Suzuki K.-I., Ludwig W., Whitman W. B.. New York: Springer;[CrossRef]
    [Google Scholar]
  12. Kelly K. L.. 1964; Inter-Society Color Council-National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Published in US Washington, DC: US Government Printing Office;
    [Google Scholar]
  13. Kim S. B., Brown R., Oldfield C., Gilbert S. C., Iliarionov S., Goodfellow M.. 2000; Gordonia amicalis sp. nov., a novel dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol50:2031–2036 [CrossRef][PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H.. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes. Inter J Sys Bacteriol20:435–443 [CrossRef]
    [Google Scholar]
  17. Mandel M., Marmur J.. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol12B:195–206[CrossRef]
    [Google Scholar]
  18. McKerrow J., Vagg S., McKinney T., Seviour E. M., Maszenan A. M., Brooks P., Seviour R. J.. 2000; A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol30:178–182 [CrossRef][PubMed]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  21. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R.. 1994; Phenotypic characterizationMethods for general and molecular bacteriology. In American Society for Microbiology , pp.607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  24. Waksman S. A.. 1967; The Actinomycetes. A Summary of Current Knowledge New York, NY: Ronald Press;
    [Google Scholar]
  25. Waksman S. A., Henrici A. T.. 1943; The Nomenclature and classification of the actinomycetes. J Bacteriol46:337–341[PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E.. 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  27. Wellington E. M., Stackebrandt E., Sanders D., Wolstrup J., Jorgensen N. O.. 1992; Taxonomic status of Kitasatosporia, and proposed unification with Streptomyces on the basis of phenotypic and 16S rRNA analysis and emendation of Streptomyces Waksman and Henrici 1943, 339AL. Int J Syst Bacteriol42:156–160 [CrossRef][PubMed]
    [Google Scholar]
  28. Witt D., Stackebrandt E.. 1990; Unification of the genera streptoverticillum and streptomyces, and amendation of streptomyces waksman and henrici 1943, 339AL. Int J Syst Bacteriol13:361–371 [CrossRef]
    [Google Scholar]
  29. Wu C., Lu X., Qin M., Wang Y., Ruan J.. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English Translation of Microbiology (Beijing)]16:176–178
    [Google Scholar]
  30. Xiang W., Liu C., Wang X., Du J., Xi L., Huang Y.. 2011; Actinoalloteichus nanshanensis sp. nov., isolated from the rhizosphere of a fig tree (Ficus religiosa). Int J Syst Evol Microbiol61:1165–1169 [CrossRef][PubMed]
    [Google Scholar]
  31. Xie Q. Y., Lin H. P., Li L., Brown R., Goodfellow M., Deng Z., Hong K.. 2012; Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. Antonie Van Leeuwenhoek102:1–7 [CrossRef][PubMed]
    [Google Scholar]
  32. Yokota A., Tamura T., Hasegawa T., Huang L. H.. 1993; Catenuloplanes japonicus gen. nov., sp. nov., nom. rev., a new genus of the order actinomycetales. Int J Sys Bacteriol43:805–812 [CrossRef]
    [Google Scholar]
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