1887

Abstract

A novel single-spore-producing actinomycete, designated strain 2H-TWYE14, was isolated from the head of an ant (Camponotus japonicus Mayr) and characterized using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain 2H-TWYE14 belongs to the genus Streptomyces , with highest sequence similarity to Streptomyces niveus NRRL 2466 (98.84 %). Analysis based on the gyrB gene also indicated that strain 2H-TWYE14 should be assigned to the genus Streptomyces . The chemotaxonomic properties of strain 2H-TWYE14 were consistent with those of members of the genus Streptomyces . The cell wall contained ll-diaminopimelic acid. The predominant menaquinones were MK-9(H6), MK-9(H8) and MK-9(H4). The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major fatty acids were iso-C16 : 0 and iso-C15 : 0. DNA–DNA hybridization experiments and phenotypic tests were carried out between strain 2H-TWYE14 and its phylogenetically closely related strain S. niveus JCM 4251, which further clarified their relatedness and demonstrated that 2H-TWYE14 could be distinguished from S. niveus. Therefore, it is concluded that strain 2H-TWYE14 can be classified as representing a novel species of the genus Streptomyces , for which the name Streptomyces camponoticapitis sp. nov. is proposed. The type strain is 2H-TWYE14 (=DSM 100523=CGMCC 4.7275).

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2016-10-01
2019-10-23
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