1887

Abstract

A filamentous actinomycete, designated strain 1SS5-02, was isolated from mangrove sediment collected from Ranong province, Thailand. The strain formed aerial and substrate mycelia composed of long, branched hyphae. Aerial mycelia differentiated into non-motile, rod-shaped spores. The organism contained -diaminopimelic acid and no diagnostic sugars in whole-cell hydrolysates. The predominant menaquinones were MK-11(H), MK-11(H) and MK-11(H). Polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. The major fatty acids were iso-C, C 9, 10-methyl C and anteiso-C. The G+C content of the genomic DNA was 73.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1SS5-02 belonged to the genus . The strain showed the highest degree of 16S rRNA gene sequence similarity with ‘’ HA11166 (97.9 %) and VKM Ac-942 (97.8 %). However, strain 1SS5-02 could be distinguished from its nearest phylogenetic relatives in the genus on the basis of DNA–DNA relatedness values and the combination of phenotypic properties. On the basis of polyphasic taxonomy, strain 1SS5-02 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1SS5-02 (=BCC 75410=NBRC 110934).

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2016-10-01
2021-07-26
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