1887

Abstract

A filamentous actinomycete, designated strain 1SS5-02, was isolated from mangrove sediment collected from Ranong province, Thailand. The strain formed aerial and substrate mycelia composed of long, branched hyphae. Aerial mycelia differentiated into non-motile, rod-shaped spores. The organism contained -diaminopimelic acid and no diagnostic sugars in whole-cell hydrolysates. The predominant menaquinones were MK-11(H), MK-11(H) and MK-11(H). Polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and four unidentified lipids. The major fatty acids were iso-C, C 9, 10-methyl C and anteiso-C. The G+C content of the genomic DNA was 73.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1SS5-02 belonged to the genus . The strain showed the highest degree of 16S rRNA gene sequence similarity with ‘’ HA11166 (97.9 %) and VKM Ac-942 (97.8 %). However, strain 1SS5-02 could be distinguished from its nearest phylogenetic relatives in the genus on the basis of DNA–DNA relatedness values and the combination of phenotypic properties. On the basis of polyphasic taxonomy, strain 1SS5-02 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1SS5-02 (=BCC 75410=NBRC 110934).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001273
2016-10-01
2019-12-11
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3835.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001273&mimeType=html&fmt=ahah

References

  1. Al-Zarban S. S., Abbas I., Al-Musallam A. A., Steiner U., Stackebrandt E., Kroppenstedt R. M.. 2002; Nocardiopsis halotolerans sp. nov., isolated from salt marsh soil in Kuwait. Int J Syst Evol Microbiol52:525–529 [CrossRef][PubMed]
    [Google Scholar]
  2. Bouras N., Meklat A., Zitouni A., Mathieu F., Schumann P., Spröer C., Sabaou N., Klenk H. P.. 2015; Nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from saharan soil. Antonie Van Leeuwenhoek107:313–320 [CrossRef][PubMed]
    [Google Scholar]
  3. Chun J., Bae K. S., Moon E. Y., Jung S. O., Lee H. K., Kim S. J.. 2000; Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol50:1909–1913 [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100:221–230 [CrossRef][PubMed]
    [Google Scholar]
  5. Cross T., Maciver A. M., Lacey J.. 1968; The thermophilic actinomycetes in mouldy hay: Micropolyspora faeni sp.nov. J Gen Microbiol50:351–359 [CrossRef][PubMed]
    [Google Scholar]
  6. Evtushenko L., Taran V., Akimov V. N., Kroppenstedt R. M., Tiedje J. M., Stackebrandt E.. 2000; Nocardiopsis tropica sp. nov., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsis dassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol50:73–81 [CrossRef][PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229 [CrossRef]
    [Google Scholar]
  8. Fang C., Zhang J., Pang H., Li Y., Xin Y., Zhang Y.. 2011; Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment. Int J Syst Evol Microbiol61:2640–2645 [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution39:783–789 [CrossRef]
    [Google Scholar]
  10. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416 [CrossRef]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. 1974; Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24:54–63 [CrossRef]
    [Google Scholar]
  12. Hamedi J., Mohammadipanah F., Pötter G., Spröer C., Schumann P., Göker M., Klenk H. P.. 2011; Nocardiopsis arvandica sp. nov., isolated from sandy soil. Int J Syst Evol Microbiol61:1189–1194 [CrossRef][PubMed]
    [Google Scholar]
  13. Hasegawa T., Takizawa M., Tanida S.. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen App Microbiol29:319–322 [CrossRef]
    [Google Scholar]
  14. Hozzein W. N., Trujillo M. E.. 2012; Genus I. Nocardiopsis. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. , Phylum XXVI. Actinobacteria phyl. nov pp1891–1906 Edited by Whitman W. B.. New York: Springer;
    [Google Scholar]
  15. Huang H. Q., Xing S. S., Yuan W. D., Wang Y., Liu M., Sun Q. G., Lin X. Z., Bao S. X.. 2015; Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment. Antonie Van Leeuwenhoek107:1541–1546 [CrossRef][PubMed]
    [Google Scholar]
  16. Jacobson E., Grauville W. C., Fogs C. E.. 1958; Color Harmony Manual, 4th edn Chicago: Container Corporation of America;
    [Google Scholar]
  17. Kieser T., Bibb M. J., Buttner M., Chater K. F., Hopwood D. A.. 2000; Practical Streptomyces Genetics Norwich: The John Innes Foundation;
    [Google Scholar]
  18. Kimura M.. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  19. Kroppenstedt R. M., Evtushenko L. I.. 2006; The family Nocardiopsaceae. In The Prokaryotes. a Handbook on the Biology of Bacteriavol. 3, 3rd edn. pp754–795 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E.. New York: Springer;
    [Google Scholar]
  20. Küster E., Williams S. T.. 1964; Selective media for the isolation of streptomycetes. Nature202:928–929[CrossRef]
    [Google Scholar]
  21. Lechevalier H. G., Lechevalier M. P.. 1970; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales , pp.393–405 Edited by Prauser H.. Jena: Gustav Fischer;
    [Google Scholar]
  22. Lechevalier M. P., De Bievre C., Lechevalier H.. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol5:249–260 [CrossRef]
    [Google Scholar]
  23. Li W. J., Kroppenstedt R. M., Wang D., Tang S. K., Lee J. C., Park D. J., Kim C. J., Xu L. H., Jiang C. L.. 2006; Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol56:1089–1096 [CrossRef][PubMed]
    [Google Scholar]
  24. Meyer J.. 1976; Nocardiopsis, a new genus of the order actinomycetales. Int J Syst Bacteriol26:487–493 [CrossRef]
    [Google Scholar]
  25. Meyer J.. 1994; The Genus Nocardiopsis Meyer 1976. In Bergey’s Manual of Determinative Bacteriology9th edn pp2562–2568 Edited by Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  26. Mingma R., Pathom-aree W., Trakulnaleamsai S., Thamchaipenet A., Duangmal K.. 2014; Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen, Xanthomonas campestris pv. glycine. World J Microbiol Biotechnol30:271–280 [CrossRef][PubMed]
    [Google Scholar]
  27. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M.. 1980; Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr188:221–233 [CrossRef]
    [Google Scholar]
  28. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241 [CrossRef]
    [Google Scholar]
  29. Pan H. Q., Zhang D. F., Li L., Jiang Z., Cheng J., Zhang Y. G., Wang H. F., Hu J. C., Li W. J.. 2015; Nocardiopsis oceani sp. nov. and Nocardiopsis nanhaiensis sp. nov., actinomycetes isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol65:3384–3391 [CrossRef][PubMed]
    [Google Scholar]
  30. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol46:1088–1092 [CrossRef][PubMed]
    [Google Scholar]
  31. Sabry S. A., Ghanem N. B., Abu-Ella G. A., Schumann P., Stackebrandt E., Kroppenstedt R. M.. 2004; Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol54:453–456 [CrossRef][PubMed]
    [Google Scholar]
  32. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4:406–425[PubMed]
    [Google Scholar]
  33. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 North Newwark, DE: MIDI Inc;
    [Google Scholar]
  34. Shirling E. B., Gottlieb D.. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol16:313–340 [CrossRef]
    [Google Scholar]
  35. Staneck J. L., Roberts G. D.. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol28:226–231[PubMed]
    [Google Scholar]
  36. Tamaoka J., Komagata K.. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett25:125–128 [CrossRef]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. 2011; meg a5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  39. Uchida K., Kudo T., Suzuki K. I., Nakase T.. 1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol45:49–56 [CrossRef][PubMed]
    [Google Scholar]
  40. Waksman S. A.. 1961; The Actinomycetes, Vol. II. Classification, Identification and Descriptions of Genera and Species Baltimore: Williams & Wilkins;
    [Google Scholar]
  41. Waksman S. A.. 1967; The Actinomycetes. A Summary of Current Knowledge New York: Ronald Press;
    [Google Scholar]
  42. Wayne L. G., Brenner D. J., Colwell R. R. et al. 1987; International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  43. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J.. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol129:1743–1813 [CrossRef][PubMed]
    [Google Scholar]
  44. Zhang Z., Wang Y., Ruan J.. 1998; Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol48:411–422 [CrossRef][PubMed]
    [Google Scholar]
  45. Zhang Y. G., Liu Q., Wang H. F., Park D. J., Guo J. W., Kim C. J., Zhang Y. M., Li W. J.. 2015; Nocardiopsis ansamitocini sp. nov., a new producer of ansamitocin P-3 of the genus Nocardiopsis. Int J Syst Evol Microbiol66:230–235 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001273
Loading
/content/journal/ijsem/10.1099/ijsem.0.001273
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error