subsp. subsp. nov., lactic acid bacteria isolated from wild gaur () dung, and description of subsp. subsp. nov. Free

Abstract

A taxonomic investigation was performed on a Gram-stain-positive coccus, designated strain BSN307, isolated from gaur (Indian bison, ) dung based on phenotypic and molecular approaches. Based on the biochemical tests, cellular morphology and 16S rRNA gene sequence similarity, this strain was found to be a member of the genus and closely related to ATCC 49156 (99.6 % 16S rRNA gene sequence similarity) and 516 (99.0 %). However, DNA–DNA hybridization studies showed that the level of relatedness between strain BSN307 and ATCC 49156 was 75.8 %, suggesting that it represented a novel subspecies of The inability to grow in brain heart infusion (BHI) medium at pH 9.6, in tryptic soy agar (TSA) with 4 % (w/v) NaCl and at 42 °C (MRS agar) clearly differentiated BSN307 from ATCC 49156. Rep-PCR fingerprint patterns, substantial differences in summed feature 8 (C 7/C 6), C cyclo 8 and C also differentiated strain BSN307 from the reference strain of . Moreover, analysis of the housekeeping genes and revealed sequence similarities that were at the limit for species differentiation (92.2 and 97.8 %, respectively). Combined genotypic and phenotypic data indicate that strain BSN307 represents a subspecies of for which the name subsp. subsp. nov. is proposed. The type strain is BSN307 (=DSM 100577 =MCC 2824=KCTC 21083).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001268
2016-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/10/3805.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001268&mimeType=html&fmt=ahah

References

  1. Cai Y., Yang J., Pang H., Kitahara M. 2011; Lactococcus fujiensis sp. nov., a lactic acid bacterium isolated from vegetable matter. Int J Syst Evol Microbiol 61:1590–1594 [View Article][PubMed]
    [Google Scholar]
  2. Chen Y. S., Otoguro M., Lin Y. H., Pan S. F., Ji S. H., Yu C. R., Liou M. S., Chang Y. C., Wu H. C., Yanagida F. 2014; Lactococcus formosensis sp. nov., a lactic acid bacterium isolated from yan-tsai-shin (fermented broccoli stems). Int J Syst Evol Microbiol 64:146–151 [View Article][PubMed]
    [Google Scholar]
  3. Cho S. L., Nam S. W., Yoon J. H., Lee J. S., Sukhoom A., Kim W. 2008; Lactococcus chungangensis sp. nov., a lactic acid bacterium isolated from activated sludge foam. Int J Syst Evol Microbiol 58:1844–1849 [View Article][PubMed]
    [Google Scholar]
  4. Collins M. D., Farrow J. A., Phillips B. A., Kandler O. 1983; Streptococcus garvieae sp. nov. and Streptococcus plantarum sp. nov. J Gen Microbiol 129:3427–3431 [View Article][PubMed]
    [Google Scholar]
  5. Edgar R. C. 2004; MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  6. Eldar A., Goria M., Ghittino C., Zlotkin A., Bercovier H. 1999; Biodiversity of Lactococcus garvieae strains isolated from fish in Europe, Asia, and Australia. Appl Environ Microbiol 65:1005–1008[PubMed]
    [Google Scholar]
  7. Fernandez E., Alegria A., Delgado S., Mayo B. 2010; Phenotypic, genetic and technological characterization of Lactococcus garvieae strains isolated from a raw milk cheese. Int Dairy J 20:142–148 [CrossRef]
    [Google Scholar]
  8. Gillis M., De Ley J., De Cleene M. 1970; The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153 [View Article][PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4:770–773[PubMed] [CrossRef]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  12. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. 1992 International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Ley J. D., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  14. Loveland-Curtze J., Miteva V. I., Brenchley J. E. 2011; Evaluation of a new fluorimetric DNA-DNA hybridization method. Can J Microbiol 57:250–255 [View Article][PubMed]
    [Google Scholar]
  15. Meier-Kolthoff J. P., Hahnke R. L., Petersen J., Scheuner C., Michael V., Fiebig A., Rohde C., Rohde M., Fartmann B. et al. 2014; Complete genome sequence of DSM 30083(T), the type strain (U5/41(T)) of Escherichia coli, and a proposal for delineating subspecies in microbial taxonomy. Stand Genomic Sci 9:2 [View Article][PubMed]
    [Google Scholar]
  16. Meucci A., Zago M., Rossetti L., Fornasari M. E., Bonvini B., Tidona F., Povolo M., Contarini G., Carminati D., Giraffa G. 2015; Lactococcus hircilactis sp. nov. and Lactococcus laudensis sp. nov., isolated from milk. Int J Syst Evol Microbiol 65:2091–2096 [View Article][PubMed]
    [Google Scholar]
  17. Naser S. M., Dawyndt P., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Vancanneyt M., Swings J. 2007; Identification of lactobacilli by pheS and rpoA gene sequence analyses. Int J Syst Evol Microbiol 57:2777–2789 [View Article][PubMed]
    [Google Scholar]
  18. Pérez T., Balcázar J. L., Peix A., Valverde A., Velázquez E., De Blas I., Ruiz-Zarzuela I. 2010; Lactococcus lactis subsp. tructae subsp. nov. isolated from the intestinal mucus of brown trout (Salmo trutta) and rainbow trout (Oncorhynchus mykiss) . Int J Syst Evol Microbiol 61:1894–1898 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Schleifer K. H., Kraus J., Dvorak C., Kilpper-Balz R., Collins M. D., Fischer W. 1985; Transfer of Streptococcus lactis and related Streptococci to the genus Lactococcus gen. nov. Syst Appl Microbiol 6:183–195 [View Article]
    [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  22. Varsha K. K., Devendra L., Shilpa G., Priya S., Pandey A., Nampoothiri K. M. 2015; 2,4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp. Int J Food Microbiol 211:44–50 [View Article][PubMed]
    [Google Scholar]
  23. Varsha K., Priya S., Devendra L., Nampoothiri K. 2014; Control of spoilage fungi by protective lactic acid bacteria displaying probiotic properties. Appl Biochem Biotechnol1–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001268
Loading
/content/journal/ijsem/10.1099/ijsem.0.001268
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed