1887

Abstract

Two Gram-stain-positive strains, VE80 and VE116, which were resistant to vancomycin, were isolated from retail chicken meat and liver in Ho Chi Minh, Vietnam, respectively. These strains were characterized by sequence analyses of 16S rRNA, RNA polymerase α-subunit (), ATP synthase α-subunit (), and phenylalanyl-tRNA synthase α-subunit () genes, determination of DNA G+C content, cellular fatty acid methyl ester analysis, DNA–DNA hybridization, and conventional morphological and biochemical tests. Strains VE80 and VE116 had 99.6 % 16S rRNA gene sequence similarity with LMG 13590, and 99.1 % 16S rRNA gene sequence similarity with ATCC 51266. However, the two isolates could be clearly differentiated from these reference strains by the low sequence similarities (86.1–86.8 %) of the gene, low DNA–DNA relatedness (<22.8 %), and differences in the production of acid from melezitose and methyl α--glucoside. Based on the results obtained in the present study, these two isolates are considered to represent a novel species of the genus for which the name sp. nov., is proposed. The type strain is VE80 (=JCM 31193=CCUG 68827).

Keyword(s): chicken and Enterococcus
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2016-10-01
2021-07-29
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