@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.001262, author = "Gao, Jun-lian and Sun, Pengbo and Wang, Xu-ming and Cheng, Shoutao and Lv, Fanyang and Qiu, Tian-lei and Yuan, Mei and Sun, Jian-guang", title = "Sphingomonaszeicaulis sp. nov., an endophytic bacterium isolated from maize root", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "9", pages = "3755-3760", doi = "https://doi.org/10.1099/ijsem.0.001262", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001262", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Sphingomonas zeicaulis", keywords = "endophytic bacterium", keywords = "maize", keywords = "root", abstract = "A novel Gram-staining-negative, aerobic and rod-shaped strain designated 541T was isolated from surface-sterilized root tissue of maize, collected from the Fangshan District of Beijing, People’s Republic of China, and was subjected to a taxonomic study using a polyphasic approach. According to a phylogenetic tree based on 16S rRNA gene sequences, strain 541T represented a member of the genus Sphingomonas and clustered with Sphingomonas sanxanigenens DSM 19645T, with which it shared the highest 16S rRNA gene sequence similarity (98.8 %). The predominant respiratory quinone was ubiquinone-10 (Q-10), the major polyamine was sym-homospermidine and the major cellular fatty acids were C18 : 1ω7c (50.9 %), C16 : 0 (22.0 %) and C14 : 0 2-OH (11.4 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and sphingoglycolipid. The DNA G+C content was 64.7 mol%. DNA–DNA relatedness between strain 541T and its closest phylogenetic relative Sphingomonas sanxanigenens DSM 19645T was 50.8 %. The results of physiological and biochemical tests and the differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain 541T from closely related species of the genus Sphingomonas . Strain 541T represents a novel species within the genus Sphingomonas , for which the nameSphingomonas zeicaulis sp. nov. is proposed, with the type strain 541T (=CGMCC 1.15008T=DSM 100587T).", }