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Abstract

Novel Gram-stain-variable, bent rods or long filaments that were endospore-forming, facultatively anaerobic, oxidase- and catalase-negative, and designated strain NC2-42, were isolated from sediment on the coast of Weihai, China. Optimal growth occurred at 37 °C, pH 7.5 and with 2–3 % (w/v) NaCl. MK-7 was the sole respiratory quinone and -diaminopimelic acid was a diagnostic diamino acid in the peptidoglycan. The polar lipid profile of this novel isolate consisted of phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid, an unknown phosphoaminolipid, two unknown glycolipids and an unknown lipid. The major cellular fatty acids in strain NC2-42 were anteiso-C, iso-C and C. The G+C content of the genomic DNA of strain NC2-42 was 58.11  mol% (HPLC). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NC2-42 showed the highest similarity (92.32 %) to within the family . Based on data from this taxonomic study using a polyphasic approach, the isolate is proposed to represent a novel species of a new genus within the family , with the name gen. nov., sp. nov. The type strain of the type species is NC2-42 (=KCTC 33676=MCCC 1K01238).

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2016-09-01
2020-01-24
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