1887

Abstract

Novel Gram-stain-variable, bent rods or long filaments that were endospore-forming, facultatively anaerobic, oxidase- and catalase-negative, and designated strain NC2-42, were isolated from sediment on the coast of Weihai, China. Optimal growth occurred at 37 °C, pH 7.5 and with 2–3 % (w/v) NaCl. MK-7 was the sole respiratory quinone and -diaminopimelic acid was a diagnostic diamino acid in the peptidoglycan. The polar lipid profile of this novel isolate consisted of phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid, an unknown phosphoaminolipid, two unknown glycolipids and an unknown lipid. The major cellular fatty acids in strain NC2-42 were anteiso-C, iso-C and C. The G+C content of the genomic DNA of strain NC2-42 was 58.11  mol% (HPLC). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NC2-42 showed the highest similarity (92.32 %) to within the family . Based on data from this taxonomic study using a polyphasic approach, the isolate is proposed to represent a novel species of a new genus within the family , with the name gen. nov., sp. nov. The type strain of the type species is NC2-42 (=KCTC 33676=MCCC 1K01238).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001257
2016-09-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3725.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001257&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genusPaenibacillus . Antonie van Leeuwenhoek 64:253–260[PubMed] [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 56:781–786
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1974; Bacterial characters and characterization. In Cowan and Steel’s Manual for the Identification of Medical Bacteria, 2nd edn. Edited by Cowan S. T. Cambridge, UK: Cambridge University Press;
    [Google Scholar]
  4. De Vos P., Ludwig W., Schleifer K.-H., Whitman W. B. 2009; Family IV. Paenibacillaceae fam. nov. In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 3 pp. 269–280 . Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. New York: Springer;
    [Google Scholar]
  5. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Scientific Press;
    [Google Scholar]
  6. Felsenstein J. 2005 PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA
  7. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [View Article]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of the protein molecules. In Mammalian Protein Metabolism , pp. 21–132 Edited by Munro H. N. New York: Academic Press; [CrossRef]
    [Google Scholar]
  9. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B. J. 2006; Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56:781–786 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics , pp. 115–147 Edited by Goodfellow M., Stackebrandt E. UK: Wiley;
    [Google Scholar]
  12. Lee F. L., Kuo H. P., Tai C. J., Yokota A., Lo C. C. 2007; Paenibacillus taiwanensis sp. nov., isolated from soil in Taiwan. Int J Syst Evol Microbiol 57:1351–1354 [View Article][PubMed]
    [Google Scholar]
  13. Marchesi J. R., Sato T., Weightman A. J., Martin T. A., Fry J. C., Hiom S. J., Dymock D., Wade W. G. 1998; Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795–799[PubMed]
    [Google Scholar]
  14. Mayilraj S., Stackebrandt E. 2014; The family Paenibacillaceae . In The Prokaryotes, 4th edn. pp 267–280 Edited by Rosenberg E., DeLong E. F., Lory S., Stackebrandt E., Thompson. F. Berlin:: Springer;
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  16. Pruesse E., Peplies J., Glöckner F. O. 2012; SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 28:1823–1829 [View Article][PubMed]
    [Google Scholar]
  17. Saha P., Krishnamurthi S., Bhattacharya A., Sharma R., Chakrabarti T. 2010; Fontibacillus aquaticus gen. nov., sp. nov., isolated from a warm spring. Int J Syst Evol Microbiol 60:422–428 [View Article][PubMed]
    [Google Scholar]
  18. Schumann P. 2011; Peptidoglycan structure. Methods Microbiol 38:101–129 [CrossRef]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology , pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Stamatakis A. 2014; RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313 [View Article][PubMed]
    [Google Scholar]
  21. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R. 2007; Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology, 3rd edn. , pp. 330–393 Edited by Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Touzel J. P., Prensier G. 2009; Genus VII Thermobacillus . In Bergey’s manual of systematic bacteriology, 2nd edn. vol. 3 , pp. 321–322 Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey E. A., Schleifer K. H., Withman W. B. Dordrecht/Heidelberg/London/ New York: Springer;
    [Google Scholar]
  23. Westram R., Bader K., Pruesse E., Kumar Y., Meier H., Glöckner F. O., Ludwig W. 2011; ARB: a software environment for sequence data. In Handbook of Molecular Microbial Ecology I: Metagenomics and Complementary Approaches pp. 399–406 Edited by De Bruijn F. J. Hoboken, NJ:: John Wiley & Sons; [CrossRef]
    [Google Scholar]
  24. Yarza P., Richter M., Peplies J., Euzeby J., Amann R., Schleifer K. H., Ludwig W., Glöckner F. O., Rosselló-Móra R. 2008; The All-Species Living Tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31:241–250 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001257
Loading
/content/journal/ijsem/10.1099/ijsem.0.001257
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error