1887

Abstract

A novel bacterial strain, designated strain BS27, was isolated from mushroom compost and subjected to a taxonomic study using a polyphasic approach. Colonies of BS27 were milky-white, circular with regular fringes and opaque. Cells were short rods, 0.3–0.5 µm wide and 1.2–2.0 µm long. Phylogenetic study based on the 16S rRNA gene sequence placed BS27 in a distinct lineage in the family , sharing 90.1–90.9 % sequence similarity with members of the closely related genera , , , and . The novel isolate showed the highest sequence similarities with the members of the genus . BS27 contained MK-7 as predominant quinone, and iso-C, iso-C 3-OH, Cω7 and/or Cω6 (summed feature 3) and iso-CI and/or anteiso-CB (summed feature 4) as major fatty acids. The DNA G+C content was 53.0 mol%. The major polar lipids of BS27 were phosphatidylethanolamine (PE) and five unidentified polar lipids (L1, L2, L5, L6 and L7). The results of physiological and biochemical tests allowed phenotypic differentiation of BS27 from its closest phylogenetic neighbours. On the basis of the evidence of this polyphasic study, isolate BS27 represents a novel genus and species in the family for which the name gen. nov., sp. nov. is proposed. The type strain is BS27 (=KACC 18745=JCM 17664).

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2016-09-01
2021-08-06
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