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Abstract

A Gram-staining-negative, aerobic, yellow–orange-pigmented, rod-shaped bacterium designated D-24 was isolated from seawater from sandy shoreline in Johor, Malaysia. The 16S rRNA gene sequence analysis revealed that strain D-24 is affiliated with the genus . It shared more than 96 % sequence similarity with the types of some of the validly published species of the genus: KMM 3516 (99.5 %), RSSK-12 (97.3 %), CC-CZW007 (96.9 %), VBW088 (96.7 %) and JCM 15496 (96.3 %). DNA–DNA hybridization and genome-based analysis of average nucleotide identity (ANI) of strain D-24 versus KMM 3516 exhibited values of 35.9±0.14 % and 89.26 %, respectively. Strain D-24 showed an even lower ANI value of 80.88 % with RSSK-12. The major menaquinone of strain D-24 was MK-6, and the predominant fatty acids were iso-C and iso-C 3-OH. Strain D-24 contained major amounts of phosphatidylethanolamine, two lipids and two aminolipids, and a phosphoglycolipid that was different to that of other species of the genus . The genomic DNA G+C content was 40.6 mol%. On the basis of phenotypic properties, DNA–DNA relatedness, ANI value and chemotaxonomic analyses, strain D-24 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is D-24 (=KCTC 42708=DSM 101732).

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2016-09-01
2022-08-19
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