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Abstract

A taxonomic study was carried out on strain 19-m-6, which was isolated from deep sea sediment of the South China Sea during the screening of alkane-degrading bacteria. The isolate was Gram-reaction-negative, and oxidase- and catalase- positive. On the basis of 16S rRNA gene sequence similarity, strain 19-m-6 was shown to belong to the genus , related to T9 (97.5 %), A-11-3 (97.3  %), SK2 (96.6 %) and seven other species of the genus (93.9–95.4 %). Average nucleotide identity values between strain 19-m-6 and T9, A-11-3 and SK2 were 85.12, 85.87 and 84.35 %, respectively. The estimated DNA–DNA hybridization values between strain 19-m-6 and these three type strains were 22.0, 22.6 and 21.2 %, respectively. Four alkane hydroxylase () genes were obtained from the draft genome sequence. The G+C content of the chromosomal DNA was 56.44 mol%. The major fatty acids were C, C 7 and summed feature 3 (C 6 and/or C 7). The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, one aminolipid, three phospholipids, two glycolipids and two aminophospholipids. According to its phenotypic features, fatty acid composition and 16S rRNA gene sequence, the novel strain fitted well into the genus , but could be clearly distinguished from all other known species described to date. The name sp. nov. is thus proposed, with 19-m-6 (=MCCC 1A05629=KCTC 52137) as the type strain.

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2016-09-01
2021-08-03
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