1887

Abstract

An actinobacterium, designated strain CPCC 203993, was isolated from a rhizosphere soil sample collected from Heilongjiang Province, northeast China, and was characterized using a polyphasic taxonomy approach. Cells of the strain were Gram-stain-positive, non-motile and non-endospore-forming cocci. The 16S rRNA gene sequence comparison of strain CPCC 203993 with members of the genus Microlunatu s yielded 93.9 % to 97.8 % similarities. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 203993 was affiliated to the clade of the genus Microlunatus next to Microlunatus parietis DSM 22083, while the DNA–DNA hybridization value of 31.5 % (±1.8 %) between strain CPCC 203993 and Microlunatus. parietis DSM 22083 was far below 70 %. This result indicated that strain CPCC 203993 represented a different genomic species from M. parietis . Chemotaxonomically, the strain contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, MK-9(H4) as the only menaquinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and one unidentified phospholipid in the polar lipids extracts, and anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, without mycolic acids. The genomic DNA G+C content was 64.04 mol%. The above evidence from the polyphasic study merit the recognition of strain CPCC 203993 as a representative of a novel species of the genus Microlunatus , for which Microlunatus nigridraconis sp. nov. is proposed. The type strain is CPCC 203993 (=DSM 29529=NBRC 110715=KCTC 29689).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001241
2016-09-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/9/3614.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001241&mimeType=html&fmt=ahah

References

  1. An D. S., Im W. T., Yoon M. H..( 2008;). Microlunatus panaciterrae sp. nov. A Β-glucosidase-producing bacterium, isolated from soil in a ginseng field.. Int J Syst Evol Microbiol 58: 2734–2738.[CrossRef]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E..( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100: 221–230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Cui Y. S., Im W. T., Yin C. R., Yang D. C., Lee S. T..( 2007;). Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 57: 713–716. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A..( 1970;). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12: 133–142. [CrossRef] [PubMed]
    [Google Scholar]
  5. Felsenstein J..( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17: 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J..( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39: 783–791. [CrossRef]
    [Google Scholar]
  7. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K..( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47: 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kellogg J. A., Bankert D. A., Withers G. S., Sweimler W., Kiehn T. E., Pfyffer G. E..( 2001;). Application of the sherlock mycobacteria identification system using high-performance liquid chromatography in a clinical laboratory. . J Clin Microbiol 39: 964–970. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al.( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62: 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kimura M..( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16: 111–120. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kimura M..( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: 263 Cambridge University Press;.[CrossRef]
    [Google Scholar]
  12. Kluge A. G., Farris J. S..( 1969;). Quantitative phyletics and the evolution of Anurans. . Syst Zool 18: 1–32. [CrossRef]
    [Google Scholar]
  13. Kämpfer P., Young C. C., Busse H. J., Chu J. N., Schumann P., Arun A. B., Shen F. T., Rekha P. D..( 2010a;). Microlunatus soli sp. nov., isolated from soil. . Int J Syst Evol Microbiol 60: 824–827.[CrossRef]
    [Google Scholar]
  14. Kämpfer P., Schäfer J., Lodders N., Martin K..( 2010b;). Microlunatus parietis sp. nov., isolated from an indoor wall. . Int J Syst Evol Microbiol 60: 2420–2423. [CrossRef] [PubMed]
    [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. A..( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20: 435–443. [CrossRef]
    [Google Scholar]
  16. Lechevalier M. P., Lechevalier H. A..( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (SIM Special Publication no. 6), pp. 227–291. Edited by Society for Industrial Microbiology Dietz A., Thayer D. W.. Fairfax:: VA;.
    [Google Scholar]
  17. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L..( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57: 1424–1428. [CrossRef] [PubMed]
    [Google Scholar]
  18. Marmur J., Doty P..( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5: 109–118. [CrossRef] [PubMed]
    [Google Scholar]
  19. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H..( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2: 233–241. [CrossRef]
    [Google Scholar]
  20. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Kamagata Y..( 1995;). Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. . Int J Syst Bacteriol 45: 17–22. [CrossRef] [PubMed]
    [Google Scholar]
  21. Saitou N., Nei M..( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4: 406–425.[PubMed]
    [Google Scholar]
  22. Sasser M..( 1990;). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. Technical Note, 101. Newark, DE:: Microbial Inc;.
    [Google Scholar]
  23. Shirling E. B., Gottlieb D..( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16: 313–340. [CrossRef]
    [Google Scholar]
  24. Stackebrandt E., Goebel B. M..( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Evol Microbiol 44: 846–849. [CrossRef]
    [Google Scholar]
  25. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S..( 2011;). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28: 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  26. Tuo L., Li J., Liu S. W., Liu Y., Hu L., Chen L., Jiang M. G., Sun C. H..( 2016;). Microlunatus endophyticus sp. nov., an endophytic actinobacterium isolated from bark of Bruguiera sexangula. . Int J Syst Evol Microbiol 66: 481–486. [CrossRef] [PubMed]
    [Google Scholar]
  27. Waksman S. A..( 1961;). The Actinomycetes, Classification, Identification and Description of Genera and Species, vol. 2. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  28. Wang Y. X., Cai M., Zhi X. Y., Zhang Y. Q., Tang S. K., Xu L. H., Cui X. L., Li W. J..( 2008;). Microlunatus aurantiacus sp. nov., a novel actinobacterium isolated from a rhizosphere soil sample. . Int J Syst Evol Microbiol 58: 1873–1877. [CrossRef] [PubMed]
    [Google Scholar]
  29. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I, Moore L. H., Moore W. E. C., Murray R. G. E. et al.( 1987;). International committee on systematic bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37: 463–464.[CrossRef]
    [Google Scholar]
  30. Williams S. T., Goodfellow M., Alderson G., Wellington E. M., Sneath P. H., Sackin M. J..( 1983;). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129: 1743–1813. [CrossRef] [PubMed]
    [Google Scholar]
  31. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L..( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae' isolated from China. . Int J Syst Evol Microbiol 55: 1149–1153. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001241
Loading
/content/journal/ijsem/10.1099/ijsem.0.001241
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error