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Abstract

An actinobacterium, designated strain CPCC 203993, was isolated from a rhizosphere soil sample collected from Heilongjiang Province, northeast China, and was characterized using a polyphasic taxonomy approach. Cells of the strain were Gram-stain-positive, non-motile and non-endospore-forming cocci. The 16S rRNA gene sequence comparison of strain CPCC 203993 with members of the genus yielded 93.9 % to 97.8 % similarities. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 203993 was affiliated to the clade of the genus next to DSM 22083, while the DNA–DNA hybridization value of 31.5 % (±1.8 %) between strain CPCC 203993 and DSM 22083 was far below 70 %. This result indicated that strain CPCC 203993 represented a different genomic species from Chemotaxonomically, the strain contained -2,6-diaminopimelic acid as the diagnostic diamino acid, MK-9(H) as the only menaquinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and one unidentified phospholipid in the polar lipids extracts, and anteiso-C iso-C and iso-C as the major cellular fatty acids, without mycolic acids. The genomic DNA G+C content was 64.04 mol%. The above evidence from the polyphasic study merit the recognition of strain CPCC 203993 as a representative of a novel species of the genus , for which sp. nov. is proposed. The type strain is CPCC 203993 (=DSM 29529=NBRC 110715=KCTC 29689).

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2016-09-01
2021-08-03
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