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Abstract
An actinobacterium, designated strain CPCC 203993T, was isolated from a rhizosphere soil sample collected from Heilongjiang Province, northeast China, and was characterized using a polyphasic taxonomy approach. Cells of the strain were Gram-stain-positive, non-motile and non-endospore-forming cocci. The 16S rRNA gene sequence comparison of strain CPCC 203993T with members of the genus Microlunatu s yielded 93.9 % to 97.8 % similarities. In the phylogenetic tree based on 16S rRNA gene sequences, strain CPCC 203993T was affiliated to the clade of the genus Microlunatus next to Microlunatus parietis DSM 22083T, while the DNA–DNA hybridization value of 31.5 % (±1.8 %) between strain CPCC 203993T and Microlunatus. parietis DSM 22083T was far below 70 %. This result indicated that strain CPCC 203993T represented a different genomic species from M. parietis . Chemotaxonomically, the strain contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid, MK-9(H4) as the only menaquinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified glycolipids and one unidentified phospholipid in the polar lipids extracts, and anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, without mycolic acids. The genomic DNA G+C content was 64.04 mol%. The above evidence from the polyphasic study merit the recognition of strain CPCC 203993T as a representative of a novel species of the genus Microlunatus , for which Microlunatus nigridraconis sp. nov. is proposed. The type strain is CPCC 203993T (=DSM 29529T=NBRC 110715T=KCTC 29689T).
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