1887

Abstract

Members of the genus have been isolated from or detected in a wide range of animals, from non-human primates and felids to a rodent, the guinea pig. By means of selective culture, biochemical testing, Gram staining and PCR screening for the -specific internal transcribed spacer region of the rRNA operon, we isolated four strains of the genus from the oral cavity of the wild house mouse, subsp. The isolates are highly related and form a separate clade in the genus, as judged by tree analyses using either multi-locus sequence typing of ribosomal genes or core genes. One isolate, provisionally named sp. nov. (type strain AP2031=DSM 101846=CCUG 68283=LMG 29261), was studied further. Strain AP2031/ grew well . It was naturally competent, taking up DNA in a DNA uptake sequence and -dependent manner, and was amenable to genetic manipulation. These and other genomic attributes of sp. nov. make it an ideal candidate for use in developing a mouse model for studying –host interactions.

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2016-09-01
2020-04-05
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