RT Journal Article SR Electronic(1) A1 Rooney, Alejandro P. A1 Dunlap, Christopher A. A1 Flor-Weiler, Lina B.YR 2016 T1 Acinetobacter lactucae sp. nov., isolated from iceberg lettuce (Asteraceae: Lactuca sativa) JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 9 SP 3566 OP 3572 DO https://doi.org/10.1099/ijsem.0.001234 PB Microbiology Society, SN 1466-5034, AB Strain NRRL B-41902T and three closely related strains were isolated from iceberg lettuce. The strain was found to consist of strictly aerobic, Gram-stain-negative rods that formed cocci in late stationary phase. 16S rRNA gene sequence analysis showed that strain NRRL B-41902T was most closely related to species within the genera Acinetobacter , and that a grouping of it and the three other closely related strains was most closely related to the type strain of Acinetobacter pittii, which was also confirmed through a phylogenomic analysis. Moreover, in silico DNA–DNA hybridization analysis revealed a substantial amount of genomic divergence (39.1 %) between strain NRRL B-41902T and the type strain of A. pittii , which is expected if the strains represent distinct species. Further phenotypic analysis revealed that strain NRRL B-41902T was able to utilize a combination of l-serine, citraconic acid and citramalic acid, which differentiated it from other, closely related Acinetobacter species. Therefore, strain NRRL B-41902T (=CCUG 68785T) is proposed as the type strain of a novel species, Acinetobacter lactucae sp. nov., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001234