1887

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated W2-3-4 was isolated from the reservoir of a water purifier. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain W2-3-4 grew well at 25–30 °C on nutrient and R2A agars. On the basis of 16S rRNA gene sequence similarity, strain W2-3-4 was shown to belong to the family Caulobacteraceaeand to be related to Phenylobacterium conjunctum FWC21 (98.0 % sequence similarity) and Phenylobacterium haematophilum CCUG 26751 (97.2 %). Lower sequence similarities were found with the type strains of all other recognized members of the genus Phenylobacterium (95.7–97.1 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-10 and the major fatty acids were summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c), C16 : 0, C18 : 1 ω7c 11-methyl and summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c). The polar lipids were phosphatidylglycerol, an unknown phospholipid, four unknown glycolipids and three unidentified polar lipids. DNA–DNA relatedness values between strain W2-3-4and its closest phylogenetically neighbours were below 7 %. Strain W2-3-4 could be differentiated genotypically and phenotypically from recognized species of the genus Phenylobacterium . The isolate therefore represents a novel species, for which the name Phenylobacterium aquaticum sp. nov. is proposed, with the type strain W2-3-4 (=KACC 18306=LMG 28593).

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2016-09-01
2019-10-23
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