1887

Abstract

A Gram-reaction-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated W2-3-4 was isolated from the reservoir of a water purifier. This bacterium was characterized to determine its taxonomic position by using a polyphasic approach. Strain W2-3-4 grew well at 25–30 °C on nutrient and R2A agars. On the basis of 16S rRNA gene sequence similarity, strain W2-3-4 was shown to belong to the family and to be related to FWC21 (98.0 % sequence similarity) and CCUG 26751 (97.2 %). Lower sequence similarities were found with the type strains of all other recognized members of the genus (95.7–97.1 %). The G+C content of the genomic DNA was 68.7 mol%. The major respiratory quinone was Q-10 and the major fatty acids were summed feature 8 (comprising C 7 and/or C 6), C, C 7 11-methyl and summed feature 3 (comprising C 7 and/or C 6). The polar lipids were phosphatidylglycerol, an unknown phospholipid, four unknown glycolipids and three unidentified polar lipids. DNA–DNA relatedness values between strain W2-3-4and its closest phylogenetically neighbours were below 7 %. Strain W2-3-4 could be differentiated genotypically and phenotypically from recognized species of the genus . The isolate therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain W2-3-4 (=KACC 18306=LMG 28593).

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2016-09-01
2020-08-04
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References

  1. Abraham W. R., Macedo A. J., Lünsdorf H., Fischer R., Pawelczyk S., Smit J., Vancanneyt M.. 2008; Phylogeny by a polyphasic approach of the order Caulobacterales, proposal of Caulobacter mirabilis sp. nov., Phenylobacterium haematophilum sp. nov. and Phenylobacterium conjunctum sp. nov., and emendation of the genus Phenylobacterium. Int J Syst Evol Microbiol58:1939–1949 [CrossRef][PubMed]
    [Google Scholar]
  2. Atlas R. M.. 1993; Handbook of Microbiological Media Edited by Parks L. C.. Boca Raton. FL: CRC Press;
    [Google Scholar]
  3. Buck J. D.. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol44:992–993[PubMed]
    [Google Scholar]
  4. Cappuccino J. G., Sherman N.. 2002; Microbiology: A Laboratory Manual, 6th edn. Benjamin Cummings, CA: Pearson Education;
    [Google Scholar]
  5. Chu C., Yuan C., Liu X., Yao L., Zhu J., He J., Kwon S. W., Huang X.. 2015; Phenylobacterium kunshanense sp. nov., isolated from the sludge of a pesticide manufacturing factory. Int J Syst Evol Microbiol65:325–330 [CrossRef][PubMed]
    [Google Scholar]
  6. Euzéby J. P.. 1997; List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol47:590–592 [CrossRef][PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E.. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol39:224–229[CrossRef]
    [Google Scholar]
  8. Felsenstein J.. 1985; Confidence limit on phylogenies: an approach using the bootstrap. Evolution39:783–791[CrossRef]
    [Google Scholar]
  9. Fitch W. M.. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool20:406–416[CrossRef]
    [Google Scholar]
  10. Hall T. A.. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser41:95–98
    [Google Scholar]
  11. Hiraishi A., Ueda Y., Ishihara J., Mori T.. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol42:457–469[CrossRef]
    [Google Scholar]
  12. Im W. T., Kim S. Y., Liu Q. M., Yang J. E., Lee S. T., Yi T. H.. 2010; Nocardioides ginsengisegetis sp. nov., isolated from soil of a ginseng field. J Microbiol48:623–628 [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M.. 1983; The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press;[CrossRef]
    [Google Scholar]
  15. Lingens F., Blecher R., Blecher H., Blobel F., Eberspächer J., Fröhner C., Görisch H., Görisch H., Layh G.. 1985; Phenylobacterium immobile gen. nov., sp. nov., a gram-negative bacterium that degrades the herbicide chloridazon. Int J Syst Bacteriol35:26–39[CrossRef]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B.. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39:159–167[CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods2:233–241[CrossRef]
    [Google Scholar]
  18. Moore D. D., Dowhan D.. 1995; Preparation and Analysis of DNA. In Current Protocols in Molecular Biology pp2–11 Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. New York: Wiley;
    [Google Scholar]
  19. Oh Y. S., Roh D. H.. 2012; Phenylobacterium muchangponense sp. nov., isolated from beach soil, and emended description of the genus Phenylobacterium. Int J Syst Evol Microbiol62:977–983 [CrossRef][PubMed]
    [Google Scholar]
  20. Saitou N., Nei M.. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol4:406–425
    [Google Scholar]
  21. Sasser M.. 1990; Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  22. Tamura. K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Bio Evol30:2725–2729[CrossRef]
    [Google Scholar]
  23. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25:4876–4882[PubMed][CrossRef]
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol37:463–464[CrossRef]
    [Google Scholar]
  25. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol173:697–703[PubMed]
    [Google Scholar]
  26. Weon H. Y., Kim B. Y., Kwon S. W., Go S. J., Koo B. S., Stackebrandt E.. 2008; Phenylobacterium composti sp. nov., isolated from cotton waste compost in Korea. Int J Syst Evol Microbiol58:2301–2304 [CrossRef][PubMed]
    [Google Scholar]
  27. Zhang K., Han W., Zhang R., Xu X., Pan Q., Hu X.. 2007; Phenylobacterium zucineum sp. nov., a facultative intracellular bacterium isolated from a human erythroleukemia cell line K562. Syst Appl Microbiol30:207–212 [CrossRef][PubMed]
    [Google Scholar]
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