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Abstract

A Gram-stain-negative, facultatively anaerobic bacterium, designated WM3, was isolated from surface seawater collected from the East China Sea. Cells were catalase- and oxidase-positive, short rods and motile by means of a single polar flagellum. Growth occurred at 15–43 °C (optimum 37–40 C), pH 5.5–9.5 (optimum pH 6.5–7.5) and with 0.25–9.0 % (w/v) NaCl (optimum 1.0–1.5 %). Chemotaxonomic analysis showed that the respiratory quinone was ubiquinone-8, the major fatty acids included C16 : 0 (23.6 %), C18 : 1ω7c (26.2 %) and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH, 22.1 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain WM3 was most closely related to the genus Marinobacterium , sharing the highest 16S rRNA gene sequence similarity of 95.5 % with both Marinobacterium litorale KCTC 12756 and Marinobacterium mangrovicola DSM 27697. The genomic DNA G+C content of the strain WM3 was 55.8 mol%. On the basis of phenotypic, chemotaxonomic and genotypic characteristics presented in this study, strain WM3 is suggested to represent a novel species of the genus Marinobacterium , for which the name Marinobacterium zhoushanense sp. nov. is proposed. The type strain is WM3 (=KCTC 42782=CGMCC 1.15341).

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2016-09-01
2019-10-15
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