1887

Abstract

A Gram-staining-positive, aerobic, motile, rod-shaped (0.4–0.5×2.0–4.0 µm), endospore-forming bacterium, designated strain NIOT.bflm.S4, was isolated from biofilm formed on high-density polyethylene test coupons in coastal seawater. The strain required seawater for growth. It grew with 1.0–8.0 % (w/v) NaCl, at 4–45 °C and at pH 6.5–9.0, with optimum growth with 4.0–5.0 % (w/v) NaCl, at 30 °C and at pH 7.0–8.0. Phylogenetic analyses based on 16S rRNA and partial gene sequences showed that strain NIOT.bflm.S4 formed a phylogenetic lineage with P-207, the only known species of the genus and shared sequence identities of 96.9 and 83 %, respectively, with this strain. The identities of 16S rRNA and partial gene sequences with members of other related genera such as , , , , and were ≤95 and ≤78 %, respectively. The DNA G+C content of strain NIOT.bflm.S4 was 39.1 mol%. MK-7 was found as the sole isoprenoid quinone. The major polar lipids of strain NIOT.bflm.S4 were diphosphatidylglycerol, phosphatidylethanolamine and an unknown lipid. The diagnostic diamino acid of the cell-wall peptidoglycan was -diaminopimelic acid. Major cellular fatty acids were anteiso-C (27.9 %), anteiso-C (18.6 %), C (8.7 %) and iso-C (6.6 %). On the basis of phenotypic, phylogenetic and chemotaxonomic results, we propose that the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NIOT.bflm.S4 (=KACC 18456=MTCC 12376=TBRC 5831).

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2016-09-01
2020-09-22
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References

  1. Gerhardt, Murray R. G. E., Wood W. A., Krieg N. R.. editors 1994; Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  2. Glaeser S. P., McInroy J. A., Busse H. J., Kämpfer P.. 2014; Pseudogracilibacillus auburnensis gen. nov., sp. nov., isolated from the rhizosphere of Zea mays. Int J Syst Evol Microbiol64:2442–2448 [CrossRef][PubMed]
    [Google Scholar]
  3. Gonzalez J. M., Saiz-Jimenez C.. 2002; A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol4:770–773[PubMed][CrossRef]
    [Google Scholar]
  4. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  5. Komagata K., Suzuki K.. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol19:161–208[CrossRef]
    [Google Scholar]
  6. Mandic-Mulec I., Stefanic P., van Elsas J.. 2015; Ecology of Bacillaceae. Microbiol Spectrum3:TBS-0017-2013
    [Google Scholar]
  7. Marmur J.. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol3:208–218 [CrossRef]
    [Google Scholar]
  8. McWilliam H., Li W., Uludag M., Squizzato S., Park Y. M., Buso N., Cowley A. P., Lopez R.. 2013; Analysis tool web services from the EMBL-EBI. Nucleic Acids Res41:W597–W600 [CrossRef][PubMed]
    [Google Scholar]
  9. Mesbah N. M., Whitman W. B., Mesbah M.. 2011; Determination of the G+C content of prokaryotes. In Taxonomy of Prokaryotes pp299–324 Edited by Rainey F., Oren A.. Waltham, MA: Academic Press;[CrossRef]
    [Google Scholar]
  10. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol27:104–117 [CrossRef]
    [Google Scholar]
  11. Schleifer K. H.. 2009; Classification of bacteria and Archaea: past, present and future. Syst Appl Microbiol32:533–542 [CrossRef][PubMed]
    [Google Scholar]
  12. Schumann P.. 2011; Peptidoglycan structure. In Taxonomy of Prokaryotes (Methods in Microbiology)vol. 38 pp101–129 Edited by Rainey F. A., Oren. A.. Academic Press;[CrossRef]
    [Google Scholar]
  13. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  14. Thies F. L., Weishaupt A., Karch H., Hartung H. P., Giegerich G.. 1999; Cloning, sequencing and molecular analysis of the Campylobacter jejuni groESL bicistronic operon. Microbiology145:89–98 [CrossRef][PubMed]
    [Google Scholar]
  15. Verma P., Pandey P. K., Gupta A. K., Kim H. J., Baik K. S., Seong C. N., Patole M. S., Shouche Y. S.. 2011; Shewanella indica sp. nov., isolated from sediment of the Arabian Sea. Int J Syst Evol Microbiol61:2058–2064 [CrossRef][PubMed]
    [Google Scholar]
  16. Yamamoto S., Harayama S.. 1998; Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. Int J Syst Bacteriol48:813–819 [CrossRef][PubMed]
    [Google Scholar]
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